Human PIWIL4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13625-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PIWIL4 cDNA Clone Datasheet

PIWIL4 cDNA Clone Product Information

Gene Bank Ref.ID:	BC031060
cDNA Size:	2559
Gene Synonym:	HIWI2;MIWI2;PIWIL4
Source:	Human

PIWIL4 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens piwi-like 4 (Drosophila) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PIWIL4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PIWIL4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PIWIL4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGTGGAA GAGCCCGAGT GAAGGCCAGA GGCATCGCCC GCAGCCCCAG TGCCACAGAA
61       GTGGGGCGCA TCCAAGCCTC GCCATTGCCT AGATCTGTTG ATCTTAGTAA CAATGAAGCA
121      TCCTCTAGCA ATGGCTTCTT GGGAACAAGC AGGATCTCAA CCAACGATAA ATATGGGATA
181      TCTTCTGGTG ATGCTGGAAG TACCTTCATG GAAAGAGGTG TGAAAAACAA ACAGGACTTT
241      ATGGATTTGA GTATCTGTAC CAGAGAAAAA TTGGCACATG TGAGAAATTG TAAAACAGGT
301      TCCAGTGGAA TACCTGTGAA ACTGGTTACA AACCTCTTTA ACTTAGATTT TCCCCAAGAC
361      TGGCAGCTAT ACCAGTACCA TGTGACATAT ATTCCAGATT TAGCATCTAG AAGGCTGAGA
421      ATTGCTTTAC TTTATAGTCA TAGTGAACTT TCCAACAAAG CAAAAGCATT CGACGGTGCC
481      ATCCTTTTTC TGTCACAAAA GCTAGAAGAA AAGGTCACAG AGTTGTCAAG TGAAACTCAA
541      AGAGGTGAGA CTATAAAGAT GACTATCACC CTGAAGAGGG AGCTGCCATC AAGTTCTCCC
601      GTGTGCATCC AGGTCTTCAA TATCATCTTC AGAAAGATCC TCAAAAAGTT GTCCATGTAC
661      CAAATTGGAC GGAACTTCTA TAATCCTTCA GAGCCAATGG AAATTCCCCA GCACAAATTA
721      TCCCTTTGGC CTGGGTTTGC CATTTCTGTG TCATATTTTG AAAGGAAGCT CCTGTTTAGT
781      GCTGATGTGA GTTACAAAGT CCTCCGGAAT GAGACGGTTC TGGAATTCAT GACTGCTCTC
841      TGTCAAAGAA CTGGCTTGTC CTGTTTCACC CAGACGTGTG AGAAGCAGCT AATAGGGCTC
901      ATTGTCCTTA CAAGATACAA TAACAGAACC TACTCCATTG ATGACATTGA CTGGTCAGTG
961      AAGCCCACAC ACACCTTTCA GAAGCGGGAT GGCACCGAGA TCACCTATGT GGATTACTAC
1021     AAGCAGCAGT ATGATATTAC TGTATCGGAC CTGAATCAGC CCATGCTTGT TAGTCTGTTA
1081     AAGAAGAAGA GAAATGACAA CAGTGAGGCT CAGCTCGCCC ACCTGATACC TGAGCTCTGC
1141     TTTCTAACAG GGCTGACTGA CCAGGCAACA TCTGATTTCC AGCTGATGAA GGCTGTGGCT
1201     GAAAAGACAC GTCTCAGTCC TTCAGGCCGG CAGCAGCGCC TGGCCAGGCT TGTGGACAAC
1261     ATCCAGAGGA ATACCAATGC TCGCTTTGAA CTAGAGACCT GGGGACTGCA TTTTGGAAGC
1321     CAGATATCTC TGACTGGCCG GATTGTGCCT TCAGAAAAAA TATTAATGCA AGACCACATA
1381     TGTCAACCTG TGTCTGCTGC TGACTGGTCC AAGGATATTC GAACTTGCAA GATTTTAAAT
1441     GCACAGTCTT TGAATACCTG GTTGATTTTA TGTAGCGACA GAACTGAATA TGTTGCCGAG
1501     AGCTTTCTGA ACTGCTTGAG AAGAGTTGCA GGTTCCATGG GATTTAATGT GGACTACCCC
1561     AAAATCATAA AAGTACAAGA AAATCCAGCT GCATTTGTTA GAGCTATACA GCAATATGTT
1621     GATCCTGATG TTCAGCTGGT AATGTGCATT CTGCCTTCTA ATCAGAAGAC CTATTATGAT
1681     TCCATTAAAA AATATTTGAG CTCAGACTGC CCAGTCCCAA GCCAATGTGT GCTTGCTCGG
1741     ACCTTGAATA AACAGGGCAT GATGATGAGT ATCGCCACCA AGATCGCTAT GCAGATGACT
1801     TGCAAGCTCG GAGGCGAGCT GTGGGCTGTG GAAATACCTT TAAAGTCCCT GATGGTGGTC
1861     GGTATTGATG TCTGTAAAGA TGCACTCAGC AAGGACGTGA TGGTTGTTGG ATGCGTGGCA
1921     AGTGTTAACC CCAGAATCAC CAGGTGGTTT TCCCGCTGTA TCCTTCAGAG AACAATGACT
1981     GATGTTGCAG ATTGCTTGAA AGTTTTCATG ACTGGAGCAC TCAACAAATG GTACAAGTAC
2041     AATCATGATT TGCCAGCACG GATAATTGTG TACCGTGCTG GTGTAGGGGA TGGTCAGCTG
2101     AAAACACTTA TTGAATATGA AGTCCCACAG CTGCTGAGCA GTGTGGCAGA ATCCAGCTCA
2161     AATACCAGCT CAAGACTGTC GGTGATTGTG GTCAGGAAGA AGTGCATGCC ACGATTCTTT
2221     ACCGAAATGA ACCGCACTGT ACAGAACCCC CCACTTGGCA CTGTTGTGGA TTCAGAAGCA
2281     ACACGTAACG AATGGTATGA CTTTTATCTG ATCAGCCAGG TGGCCTGCCG GGGAACTGTT
2341     AGTCCTACCT ACTATAATGT CATCTATGAT GACAACGGCT TGAAGCCCGA CCATATGCAG
2401     AGACTTACAT TCAAATTGTG CCACCTGTAC TACAACTGGC CGGGCATAGT CAGTGTCCCA
2461     GCACCATGTC AGTATGCTCA CAAGCTGACC TTTCTGGTGG CACAAAGCAT TCATAAAGAA
2521     CCCAGTCTGG AATTAGCCAA CCATCTCTTC TACCTGTGA

Quality Control:cDNA sequencing results is available upon request.