Human PIP Gene cDNA Clone / ORF Clone

PIP cDNA Clone Datasheet

PIP cDNA Clone Product Information

• Gene Bank Ref.ID: BC010950
• cDNA Size: 441
• Gene Synonym: GPIP4; GCDFP15; GCDFP-15; PIP
• Source: Human

PIP cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens prolactin-induced protein DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PIP cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PIP cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PIP cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGCTTGC TCCAGCTCCT GTTCAGGGCC AGCCCTGCCA CCCTGCTCCT GGTTCTCTGC
61       CTGCAGTTGG GGGCCAACAA AGCTCAGGAC AACACTCGGA AGATCATAAT AAAGAATTTT
121      GACATTCCCA AGTCAGTACG TCCAAATGAC GAAGTCACTG CAGTGCTTGC AGTTCAAACA
181      GAATTGAAAG AATGCATGGT GGTTAAAACT TACCTCATTA GCAGCATCCC TCTACAAGGT
241      GCATTTAACT ATAAGTATAC TGCCTGCCTA TGTGACGACA ATCCAAAAAC CTTCTACTGG
301      GACTTTTACA CCAACAGAAC TGTGCAAATT GCAGCCGTCG TTGATGTTAT TCGGGAATTA
361      GGCATCTGCC CTGATGATGC TGCTGTAATC CCCATCAAAA ACAACCGGTT TTATACTATT
421      GAAATCCTAA AGGTAGAATA A

 

Quality Control:cDNA sequencing results is available upon request.