Mouse PIK3R4 Gene cDNA Clone / ORF Clone

PIK3R4 cDNA Clone Datasheet

PIK3R4 cDNA Clone Product Information

• Gene Bank Ref.ID: BC157947.1
• cDNA Size: 4077
• Gene Synonym: C85833; D9Ertd418e; 2210010O15Rik; C730038E05Rik; Pik3r4
• Source: Mouse

PIK3R4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 4, p150 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for two point mutations:11 A/G resulting in the amino acid Gln substitution by Arg, 2255 G/A resulting in the amino acid Arg substitution by Gln.

PIK3R4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PIK3R4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PIK3R4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAAATC GGCTGGCTGG TATCGCTCCT TCCCAGATCC TGTCTGTAGA GAGTTATTTC
61       TCAGACATCC ATGACTTTGA GTATGATAAG AGCCTGGGAA GCACTCGGTT TTTTAAAGTC
121      GCTCGAGCCA AGCACCGGGA AGGCCTGGTG GTTGTGAAGG TCTTTGCAAT TCAGGATCCC
181      ACGTTACCCT TAACTAGTTA TAAACAGGAG CTGGAGGAAC TGAAAATCCG GCTCCACTCC
241      GCCCAGAACT GCCTCCCCTT CCAGAAAGCA GCAGAAAAGG CGTCTGAGAA AGCAGCCATG
301      CTGTTCCGGC AGTATGTGAG AGACAACCTC TACGATCGCA TCAGTACCCG CCCGTTCCTA
361      AACAACATCG AGAAGCGCTG GATCGCCTTC CAGATCCTGA CAGCCGTGGA CCAAGCGCAC
421      AAATCTGGAG TCCGCCACGG AGACATCAAG ACAGAGAATG TGATGGTCAC CAGTTGGAAC
481      TGGGTCCTCC TAACCGACTT CGCCAGTTTT AAGCCCACAT ATCTTCCCGA GGACAACCCA
541      GCGGATTTCA ACTATTTCTT TGACACCTCG AGAAGGAGGA CATGCTACAT AGCTCCCGAG
601      CGCTTCGTTG ACGGTGGGAT GTTCGCCACC GAGTTAGAGT ACATGAGAGA CCCCTCAACC
661      CCGCTTGTCG ACCTGAATAG CAATCAGAGA GCACGGGGAG AGCTGAAGAG AGCCATGGAC
721      ATCTTTTCGG CAGGTTGCGT GATAGCTGAG CTCTTTACGG AAGGCGTACC TTTGTTTGAT
781      CTCTCTCAGC TGCTGGCGTA TAGAAATGGA CATTTTTTCC CTGAACAAGT GCTAAACAAA
841      ATTGAAGATC GCAGTATCAG AGACTTGGTA ACTCAGATGA TTAACCGTGA GCCCGAGAAG
901      CGTTTGGAAG CTGAGGACTA CCTGAAGCAG CAGCGAGGCA ACGCCTTCCC TGAGATATTT
961      TATACTTTCC TTCAGCCTTA CATGGCCCAG TTCGCCAAGG AAACCTTTCT CTCTGCAGAT
1021     GAGCGGATTT TGGTTATACG GAAAGATTTG GGCAACATTA TTCACAACCT CTGTGGACAC
1081     GATTTGCCAG AAAAAGCAGA AGGGGAGTCC AGGGCCAGTG GGCTGGTTGT CCTGGTGTCC
1141     GTGATAACGT CCTGCCTGCA GACACTGAAG TCCTGCGACT CCAAACTGGC CGCCTTGGAG
1201     CTCATTCTGC ATCTGGCCCC CAGACTGAGC GTCGAGATCC TTCTGGATCG CATCACTCCC
1261     TACCTGTTGC ATTTCAGCAA TGACTCTGTC CCAAGAGTGC GGGCTGAAGC CCTGAGGACA
1321     CTCACCAAAG TCCTTGCCCT TGTCCAAGAG GTTCCTCGCA ACGATGTCAA TATCTATCCA
1381     GAGTATATTC TCCCCGGCAT AGCCCACCTG GCCCAGGACG ACGCTACCAT CGTCAGACTG
1441     GCCTACGCTG AAAACATAGC TCTGTTGGCC GAGACGGCTC TGCGATTCCT GGAGCTTGTG
1501     CAGCTGAAAA CTCTCAACAT GGAGAACGAG CCCGATAGCG AGGAGGTAGA TGAAGCCACG
1561     CGTCCTAACG GAGACTATGA CACAGAGCTC CAGGCCTTGC ATGAGATGGT CCAGCAGAAG
1621     GTGGTCACGT TGCTAAGCGA CCCTGAGAAT ATCGTGAAAC AGACGCTGAT GGAGAGTGGG
1681     ATCACACGCT TGTGCGTCTT CTTCGGACGC CAGAAGGCCA ACGACGTTCT CTTGTCCCAC
1741     ATGATCACTT TCCTCAATGA TAAGAATGAT TGGCACCTGC GTGGAGCTTT CTTTGATAGC
1801     ATAGTCGGTG TGGCTGCCTA CGTTGGCTGG CAGAGCTCCT CCATCCTCAA GCCCCTGCTC
1861     CAGCAAGGCC TCAGCGATGC TGAGGAGTTT GTCATCGTGA AAGCTCTCAA TGCCCTGACC
1921     TGCATGTGCC AGCTAGGGCT GCTGCAGAAA CCCCATGTCT ACGAGTTCGC CAGTGATATT
1981     GCTCCCTTCC TGTGTCACCC TAACCTGTGG ATACGCTATG GCGCTGTGGG ATTTATCACG
2041     GTGGTAGCTC ATCAGATCAG TACTGCTGAT GTCTACTGCA AGCTCATGCC GTATCTCGAC
2101     CCGTATATTA CACAGCCAGT GATACAGATT GAGAGGAAGC TGGTCCTGCT CAGTGTGCTA
2161     AAGGAGCCGG TGAGCCGCTC CATATTTGAC TATGCTCTGA GGTCGAAAGA CATCGCCAGC
2221     TTGTTCAGAC ATCTCCACAT GCGCCAGAAG AAGCAAAATG GCTCTCTTCT CGACTGCCCG
2281     CCGCCTGAGG ACCCCACCAT AGCGCAACTT CTGAAGAAGC TGCTCTCACA GGGGATGACT
2341     GAAGAAGAGG AAGATAAACT TCTGGCACTG AAGGACTTCA TGATGAAATC AAATCGAGCC
2401     AAAGCTAATG CGGTAGACCA GAGCCACCTC CACGACAGCA GCCAGAAAGG TGTAATCGAC
2461     TTGGCCGCCT TAGGCATCAC TGGGAGACAA GTTGATCTTG TTAAAACCAA ACAAGAACCA
2521     GATGAGAAAC GTGCTAGGAA ACATGTGAAA CAAGACTCCA ATGTAAATGA AGAGTGGAAA
2581     AGCATGTTTG GGTCATTGGA GCCACCGAAT ATCCCACAGG CCCTGCCTAA AACCAGTGAC
2641     CATGAGGTTG TTCAGCCTGG GAAGCCACCT CGCTCTGAGT CCTCTGCTGG TATCTGTGTC
2701     CCTTTGTCAA CATCTCCACA GGTCTCAGAA GCAGCACACA TCCCGAGTAA GAAGCCTGTG
2761     ATCCCGGTTG TAAGTAGCAC GGTCCTGCCG TCCACTTACC AGATCCGGAT CACCACGTGC
2821     AAGACTGAAC TTCAGCAGCT CATACAGCAG AAGCGGGAGC AGTGCAACGC GGAGCGCATA
2881     GCCAAGCAGA TGATGGAGAA TGCCGAGTGG GAGAGCAAGC CACCTCCACC TGGGTGGCGT
2941     CCTAAAGGGC TGCTAGTTGC ACATCTTCAT GAGCACAAAT CTGCTGTGAA TCGCATCAGA
3001     GTCTCTGATG AACACTTACT TTTTGCAACA TGTTCAAATG ATGGCACAGT GAAGATCTGG
3061     AACAGCCAGA AGATGGAGGG GAAGACCACC ACAACGCGGT CTATTCTCAC GTACAGCCGA
3121     ATTGGAGGAC GAGTCAAGAC GCTAACGTTT TGCCAAGGCT CCCACTACTT GGCCATAGCA
3181     TCTGATAATG GTGCTGTCCA GCTTCTTGGA ATTGAGGCGT CTAAGTTACC CAAGTCTCCT
3241     AAAATTCACC CTCTACAAAG CAGGATTCTG GATCAGAAGG AAGATGGCTG TGTGGTGGAC
3301     ATGCATCACT TCAACTCCGG GGCACAGTCC GTTCTTGCCT ATGCCACGGT GAATGGCTCT
3361     CTGGTTGGAT GGGATCTCAG GTCTTCAAGC AACGCGTGGA CATTAAAGCA TGACCTAAAG
3421     TCAGGCCTCA TCACTTCCTT TGCTGTGGAC ATCCACCAGT GCTGGCTGTG CATAGGCACG
3481     AGCAGCGGTG CCATGGCGTG TTGGGACATG AGGTTCCAGT TGCCCATCTC CAGTCACTGT
3541     CATCCCTCCA GAGCTCGCAT CCGGCGCCTC TCCATGCACC CCCTGTACCA GTCCTGGGTA
3601     ATCGCAGCTG TTCAGGGCAA CAATGAAGTC TCCATGTGGG ACATGGAGAC TGGCGACAGG
3661     AGACTGACTC TCTGGGCCAG CAGCGCACCG CCACTGTCTG AGTTACAGCC TTCACCGCAC
3721     AGCGTCCATG GCATCTACTG CAGCCCTGCA GATGGAAACC CTATCCTGCT GACCGCTGGC
3781     TCAGACATGA AAATAAGGTT CTGGGACTTG GTTTCCCCAG AGAGGTCCTA TGTTGTTGCG
3841     GGAAGTACAG GTTCCCCGTC TGTCTCCTAC TACAAGAAGA TAATAGAAGG CACCGAGGTT
3901     GTCCAGGAAA TTCAGAATAA GCAGAAGGTC GGACCGAGTG ATGACACCCC TCGGAGGGGC
3961     CCGGAGTCTC TGCCTGTGGG ACATCATGAC ATCATTACAG ACATTGCCAC CTTCCAGACC
4021     ACTCAGGGCT TCATTGTGAC TGCTTCTAGA GACGGAATTG TGAAGGTGTG GAAATGA

 

Quality Control:cDNA sequencing results is available upon request.