Human PI3 Gene cDNA Clone / ORF Clone

PI3 cDNA Clone Datasheet

PI3 cDNA Clone Product Information

• Gene Bank Ref.ID: BC010952
• cDNA Size: 354
• Gene Synonym: ESI; WAP3; SKALP; WFDC14; cementoin;PI3
• Source: Human

PI3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens peptidase inhibitor 3, skin-derived DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PI3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PI3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PI3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGGCCA GCAGCTTCTT GATCGTGGTG GTGTTCCTCA TCGCTGGGAC GCTGGTTCTA
61       GAGGCAGCTG TCACGGGAGT TCCTGTTAAA GGTCAAGACA CTGTCAAAGG CCGTGTTCCA
121      TTCAATGGAC AAGATCCCGT TAAAGGACAA GTTTCAGTTA AAGGTCAAGA TAAAGTCAAA
181      GCGCAAGAGC CAGTCAAAGG TCCAGTCTCC ACTAAGCCTG GCTCCTGCCC CATTATCTTG
241      ATCCGGTGCG CCATGTTGAA TCCCCCTAAC CGCTGCTTGA AAGATACTGA CTGCCCAGGA
301      ATCAAGAAGT GCTGTGAAGG CTCTTGCGGG ATGGCCTGTT TCGTTCCCCA GTGA

 

Quality Control:cDNA sequencing results is available upon request.