Human PI15 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13898-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PI15 cDNA Clone Datasheet

PI15 cDNA Clone Product Information

Gene Bank Ref.ID:	BC126290
cDNA Size:	777
Gene Synonym:	P24TI; P25TI; CRISP8; DKFZp686F0366;PI15
Source:	Human

PI15 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens peptidase inhibitor 15 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PI15 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PI15 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PI15 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATAGCAA TCTCTGCCGT CAGCAGTGCA CTCCTGTTCT CCCTTCTCTG TGAAGCAAGT
61       ACCGTCGTCC TACTCAATTC CACTGACTCA TCCCCGCCAA CCAATAATTT CACTGATATT
121      GAAGCAGCTC TGAAAGCACA ATTAGATTCA GCGGATATCC CCAAAGCCAG GCGGAAGCGC
181      TACATTTCGC AGAATGACAT GATCGCCATT CTTGATTATC ATAATCAAGT TCGGGGCAAA
241      GTGTTCCCAC CGGCAGCAAA TATGGAATAT ATGGTTTGGG ATGAAAATCT TGCAAAATCG
301      GCAGAGGCTT GGGCGGCTAC TTGCATTTGG GACCATGGAC CTTCTTACTT ACTGAGATTT
361      TTGGGCCAAA ATCTATCTGT ACGCACTGGA AGATATCGCT CTATTCTCCA GTTGGTCAAG
421      CCATGGTATG ATGAAGTGAA AGATTATGCT TTTCCATATC CCCAGGATTG CAACCCCAGA
481      TGTCCTATGA GATGTTTTGG TCCCATGTGC ACACATTATA CGCAGATGGT TTGGGCCACT
541      TCCAATCGGA TAGGATGCGC AATTCATACT TGCCAAAACA TGAATGTTTG GGGATCTGTG
601      TGGCGACGTG CAGTTTACTT GGTATGCAAC TATGCCCCAA AGGGCAATTG GATTGGAGAA
661      GCACCATATA AAGTAGGGGT ACCATGTTCA TCTTGTCCTC CAAGTTATGG GGGATCTTGT
721      ACTGACAATC TGTGTTTTCC AGGAGTTACG TCAAACTACC TGTACTGGTT TAAATAA

Quality Control:cDNA sequencing results is available upon request.