PHOSPHO1 Protein (His Tag)

Phosphatase, orphan 1 Protein Datasheet

PHOSPHO1 Protein Product Information

• Synonym: PHOSPHO1
• Protein Construction: A DNA sequence encoding the human PHOSPHO1 isoform 1 (Q8TCT1-1) (Met 1-Cys 267) was expressed, with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

PHOSPHO1 Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 1
• Molecular Mass: The recombinant human PHOSPHO1 consisting of 282 amino acids and has a calculated molecular mass of 31.5 kDa. It migrates as an approximately 32 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, 10%glycerol, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PHOSPHO1 protein

PHOSPHO1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PHOSPHO1 Protein Description

PHOSPHO1 is a phosphatase whose expression is upregulated in mineralizing cells and is implicated in the generation of inorganic phosphate for matrix mineralization, a process central to skeletal development. PHOSPHO1 belongs to the haloacid dehalogenase superfamily of hydrolases and is a phosphatase highly expressed in bone. It localization is restricted to mineralizing regions of bone and growth plate and that the enzyme present within MVs is in an active state, inhibition of which decreases the capacity of MVs to mineralize. This phosphatase is capable of cleaving phosphoethanolamine (PEA) and phosphocholine to generate inorganic phosphate. PHOSPHO1 plays a role in the initiation of matrix mineralization.

References

1. Stewart AJ. et al., 2003, Protein Eng. 16 (12): 889-95.
2. Camps M. et al., 2000, FASEB J. 14 (1): 6-16.
3. Barford D. 1996, Trends Biochem Sci. 21 (11): 407-12.