Human PHKG2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10649-M	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_000294.1

cDNA Size:   1221

Synonym:  PHKG2

Source:  Human.

Description

cDNA Description: ORF Clone of Homo sapiens phosphorylase kinase, gamma 2 (testis) DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15–30 min .
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGACGCTGG ACGTGGGGCC GGAGGATGAG CTGCCCGACT GGGCCGCCGC CAAAGAGTTT
61       TACCAGAAGT ACGACCCTAA GGACGTCATC GGCAGAGGAG TGAGCTCTGT GGTCCGCCGT
121      TGTGTTCATC GAGCTACTGG CCACGAGTTT GCGGTGAAGA TTATGGAAGT GACAGCTGAG
181      CGGCTGAGTC CTGAGCAGCT GGAGGAGGTG CGGGAAGCCA CACGGCGAGA GACACACATC
241      CTTCGCCAGG TCGCCGGCCA CCCCCACATC ATCACCCTCA TCGATTCCTA CGAGTCTTCT
301      AGCTTCATGT TCCTGGTGTT TGACCTGATG CGGAAGGGAG AGCTGTTTGA CTATCTCACA
361      GAGAAGGTGG CCCTCTCTGA AAAGGAAACC AGGTCCATCA TGCGGTCTCT GCTGGAAGCA
421      GTGAGCTTTC TCCATGCCAA CAACATTGTG CATCGAGATC TGAAGCCCGA GAATATTCTC
481      CTAGATGACA ATATGCAGAT CCGACTTTCA GATTTCGGGT TCTCCTGCCA CTTGGAACCT
541      GGCGAGAAGC TTCGAGAGTT GTGTGGGACC CCAGGGTATC TAGCGCCAGA GATCCTTAAA
601      TGCTCCATGG ATGAAACCCA CCCAGGCTAT GGCAAGGAGG TCGACCTCTG GGCCTGTGGG
661      GTGATCTTGT TCACACTCCT GGCTGGCTCG CCACCCTTCT GGCACCGGCG GCAGATCCTG
721      ATGTTACGCA TGATCATGGA GGGCCAGTAC CAGTTCAGTT CCCCCGAGTG GGATGACCGT
781      TCCAGCACTG TCAAAGACCT GATCTCCAGG CTGCTGCAGG TGGATCCTGA GGCACGCCTG
841      ACAGCTGAGC AGGCCCTACA GCACCCCTTC TTTGAGCGTT GTGAAGGCAG CCAACCCTGG
901      AACCTCACCC CCCGCCAGCG GTTCCGGGTG GCAGTGTGGA CAGTGCTGGC TGCTGGACGA
961      GTGGCCCTAA GCACCCATCG TGTACGGCCA CTGACCAAGA ATGCACTGTT GAGGGACCCT
1021     TATGCGCTGC GGTCAGTGCG GCACCTCATC GACAACTGTG CCTTCCGGCT CTACGGGCAC
1081     TGGGTAAAGA AAGGGGAGCA GCAGAACCGG GCGGCTCTCT TTCAGCACCG GCCCCCTGGG
1141     CCTTTTCCCA TCATGGGCCC TGAAGAGGAG GGAGACTCTG CTGCTATAAC TGAGGATGAG
1201     GCCGTGCTTG TGCTGGGCTA G

Quality Control: cDNA sequencing results is available upon request.