Human PHGDH Gene cDNA Clone / ORF Clone

PHGDH cDNA Clone Datasheet

PHGDH cDNA Clone Product Information

• Gene Bank Ref.ID: NM_006623.3
• cDNA Size: 1602
• Gene Synonym: PDG, PGD, PGAD, PGDH, SERA, 3PGDH, 3-PGDH, MGC3017, PHGDH
• Source: Human

PHGDH cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens phosphoglycerate dehydrogenase DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1326 G/A not causing the amino acid variation.

PHGDH cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PHGDH cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PHGDH cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTTTTG CAAATCTGCG GAAAGTGCTC ATCAGTGACA GCCTGGACCC TTGCTGCCGG
61       AAGATCTTGC AAGATGGAGG GCTGCAGGTG GTGGAAAAGC AGAACCTTAG CAAAGAGGAG
121      CTGATAGCGG AGCTGCAGGA CTGTGAAGGC CTTATTGTTC GCTCTGCCAC CAAGGTGACC
181      GCTGATGTCA TCAACGCAGC TGAGAAACTC CAGGTGGTGG GCAGGGCTGG CACAGGTGTG
241      GACAATGTGG ATCTGGAGGC CGCAACAAGG AAGGGCATCT TGGTTATGAA CACCCCCAAT
301      GGGAACAGCC TCAGTGCCGC AGAACTCACT TGTGGAATGA TCATGTGCCT GGCCAGGCAG
361      ATTCCCCAGG CGACGGCTTC GATGAAGGAC GGCAAATGGG AGCGGAAGAA GTTCATGGGA
421      ACAGAGCTGA ATGGAAAGAC CCTGGGAATT CTTGGCCTGG GCAGGATTGG GAGAGAGGTA
481      GCTACCCGGA TGCAGTCCTT TGGGATGAAG ACTATAGGGT ATGACCCCAT CATTTCCCCA
541      GAGGTCTCGG CCTCCTTTGG TGTTCAGCAG CTGCCCCTGG AGGAGATCTG GCCTCTCTGT
601      GATTTCATCA CTGTGCACAC TCCTCTCCTG CCCTCCACGA CAGGCTTGCT GAATGACAAC
661      ACCTTTGCCC AGTGCAAGAA GGGGGTGCGT GTGGTGAACT GTGCCCGTGG AGGGATCGTG
721      GACGAAGGCG CCCTGCTCCG GGCCCTGCAG TCTGGCCAGT GTGCCGGGGC TGCACTGGAC
781      GTGTTTACGG AAGAGCCGCC ACGGGACCGG GCCTTGGTGG ACCATGAGAA TGTCATCAGC
841      TGTCCCCACC TGGGTGCCAG CACCAAGGAG GCTCAGAGCC GCTGTGGGGA GGAAATTGCT
901      GTTCAGTTCG TGGACATGGT GAAGGGGAAA TCTCTCACGG GGGTTGTGAA TGCCCAGGCC
961      CTTACCAGTG CCTTCTCTCC ACACACCAAG CCTTGGATTG GTCTGGCAGA AGCTCTGGGG
1021     ACACTGATGC GAGCCTGGGC TGGGTCCCCC AAAGGGACCA TCCAGGTGAT AACACAGGGA
1081     ACATCCCTGA AGAATGCTGG GAACTGCCTA AGCCCCGCAG TCATTGTCGG CCTCCTGAAA
1141     GAGGCTTCCA AGCAGGCGGA TGTGAACTTG GTGAACGCTA AGCTGCTGGT GAAAGAGGCT
1201     GGCCTCAATG TCACCACCTC CCACAGCCCT GCTGCACCAG GGGAGCAAGG CTTCGGGGAA
1261     TGCCTCCTGG CCGTGGCCCT GGCAGGCGCC CCTTACCAGG CTGTGGGCTT GGTCCAAGGC
1321     ACTACACCTG TACTGCAGGG GCTCAATGGA GCTGTCTTCA GGCCAGAAGT GCCTCTCCGC
1381     AGGGACCTGC CCCTGCTCCT ATTCCGGACT CAGACCTCTG ACCCTGCAAT GCTGCCTACC
1441     ATGATTGGCC TCCTGGCAGA GGCAGGCGTG CGGCTGCTGT CCTACCAGAC TTCACTGGTG
1501     TCAGATGGGG AGACCTGGCA CGTCATGGGC ATCTCCTCCT TGCTGCCCAG CCTGGAAGCG
1561     TGGAAGCAGC ATGTGACTGA AGCCTTCCAG TTCCACTTCT AA

 

Quality Control:cDNA sequencing results is available upon request.