Human PHF1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12246-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PHF1 cDNA Clone Datasheet

PHF1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC008834
cDNA Size:	1704
Gene Synonym:	PCL1; PHF2; MTF2L2;PHF1
Source:	Human

PHF1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens PHD finger protein 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PHF1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PHF1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PHF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGCAGC CCCCCCGGCT GAGCCGCTCT GGTGCCTCCT CACTTTGGGA CCCAGCTTCT
61       CCTGCTCCCA CCTCTGGCCC CAGGCCTCGG CTTTGGGAGG GTCAAGATGT GCTGGCCAGA
121      TGGACTGATG GGCTGCTATA CTTGGGTACC ATCAAAAAGG TGGACAGTGC TAGGGAGGTG
181      TGTCTGGTCC AGTTTGAGGA TGATTCGCAG TTTCTGGTTC TATGGAAAGA CATTAGCCCT
241      GCTGCCCTCC CTGGAGAGGA ACTCCTCTGT TGTGTCTGTC GCTCTGAGAC TGTGGTCCCT
301      GGGAACCGGC TGGTCAGCTG TGAGAAGTGT CGCCATGCTT ATCACCAGGA CTGCCATGTT
361      CCCAGGGCTC CAGCCCCTGG AGAGGGAGAG GGCACATCCT GGGTATGCCG CCAGTGTGTC
421      TTTGCGATCG CCACCAAGAG GGGAGGTGCC CTGAAGAAGG GCCCCTATGC CCGGGCCATG
481      CTGGGTATGA AGCTTTCTCT GCCATATGGA CTGAAGGGGC TGGACTGGGA TGCTGGACAT
541      CTGAGCAACC GACAGCAGAG TTACTGTTAC TGTGGTGGCC CTGGGGAGTG GAACCTGAAA
601      ATGCTGCAGT GCCGGAGCTG CCTGCAGTGG TTCCATGAGG CCTGCACCCA GTGTCTGAGC
661      AAGCCCCTCC TCTATGGGGA CAGGTTCTAT GAATTTGAAT GCTGTGTGTG TCGCGGGGGC
721      CCTGAGAAAG TCCGGAGACT ACAGCTTCGC TGGGTGGATG TGGCCCATCT TGTCCTGTAT
781      CACCTCAGTG TTTGCTGTAA GAAGAAATAC TTTGATTTTG ATCGTGAGAT CCTCCCCTTC
841      ACTTCTGAGA ATTGGGACAG TTTGCTCCTG GGGGAGCTTT CAGACACCCC CAAAGGAGAA
901      CGTTCTTCCA AGCTCCTCTC TGCTCTTAAC AGCCACAAGG ACCGTTTCAT TTCAGGGAGA
961      GAGATTAAGA AGAGGAAATG TTTGTTTGGT CTCCATGCTC GGATGCCTCC CCCTGTGGAG
1021     CCCCCTACTG GAGATGGAGC ACTCACCAGC TTCCCTTCAG GGCAGGGCCC TGGGGGAGGG
1081     GTCTCACGTC CCCTGGGGAA GCGCCGGAGG CCGGAGCCAG AGCCCCTGAG GAGGAGGCAG
1141     AAGGGGAAAG TGGAGGAGCT GGGGCCACCC TCAGCAGTGC GCAATCAGCC CGAGCCCCAG
1201     GAGCAGAGGG AGCGGGCTCA TCTGCAGAGG GCACTGCAGG CCTCAGTGTC TCCACCATCC
1261     CCCAGCCCTA ACCAGAGTTA CCAGGGCAGC AGCGGCTACA ACTTCCGGCC CACAGATGCC
1321     CGCTGCCTGC CCAGCAGCCC CATCCGGATG TTTGCTTCCT TCCACCCTTC TGCCAGCACC
1381     GCAGGGACCT CTGGGGACAG TGGACCCCCA GACAGGTCAC CCCTGGAACT TCACATTGGT
1441     TTCCCCACAG ACATCCCTAA AAGTGCCCCC CACTCGATGA CTGCCTCATC TTCCTCAGTT
1501     TCATCCCCAT CCCCAGGTCT TCCTAGACGC TCAGCACCCC CTTCTCCCCT GTGCCGTAGT
1561     TTGTCTCCTG GGACTGGGGG AGGAGTCCGA GGTGGGGTTG GTTACCTGTC CCGAGGGGAC
1621     CCTGTCCGGG TCCTTGCTCG GAGAGTACGG CCTGATGGCT CTGTGCAGTA CCTGGTTGAG
1681     TGGGGAGGAG GGGGCATCTT CTGA

Quality Control:cDNA sequencing results is available upon request.