PGDH / PHGDH Protein Price Inquiry ( Available Sizes )
PGDH / PHGDH Protein Product Information
||RP4-683H9.1, 3-PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, SERA
||A DNA sequence encoding the mature form of human PHGDH (O43175) (Met 1-Phe 533) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
PGDH / PHGDH Protein QC Testing
||> 90 % as determined by SDS-PAGE
PGDH / PHGDH protein
||Please contact us for more information.
||Samples are stable for up to twelve months from date of receipt at -70℃
|Predicted N terminal:
The recombinant human PHGDH comprises 543 amino acids and has a calculated molecular mass of 58KDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
||Lyophilized from sterile PBS,100mM Arg, 0.1% Tween20, 20%glycerol; pH8.0.
- Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
- Please contact us for any concerns or special requirements.
PGDH / PHGDH Protein Usage Guide
||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PGDH / PHGDH Protein Related Products & Topics
PGDH / PHGDH Protein Description
PHGDH belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family which is comprised by D-isomer-stereospecific enzymes. It seems that the conserved residues in the D-isomer specific 2-hydroxyacid dehydrogenase family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.
- Pind S. et al., 2002, J Biol Chem. 277 (9): 7136-43.
- Du H. et al., 2010, Reproduction. 139 (1): 237-45.
- Possemato R. et al., 2011, Nature. 476 (7360): 346-50.