PGDH / PHGDH Protein (His Tag)

Phosphoglycerate dehydrogenase Protein Datasheet

PGDH / PHGDH Protein Product Information

• Synonym: RP4-683H9.1, 3-PGDH, 3PGDH, PDG, PGAD, PGD, PGDH, SERA
• Protein Construction: A DNA sequence encoding the mature form of human PHGDH (O43175) (Met 1-Phe 533) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
• Source: Human
• Expression Host: E.coli

PGDH / PHGDH Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PHGDH comprises 543 amino acids and has a calculated molecular mass of 58KDa. It migrates as an approximately 55 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS,100mM Arg, 0.1% Tween20, 20%glycerol; pH8.0.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PGDH / PHGDH protein

PGDH / PHGDH Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PGDH / PHGDH Protein Description

PHGDH belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family which is comprised by D-isomer-stereospecific enzymes. It seems that the conserved residues in the D-isomer specific 2-hydroxyacid dehydrogenase family appear to be the residues involved in the substrate binding and the catalytic reaction, and thus to be targets for site-directed mutagenesis. A number of NAD-dependent 2-hydroxyacid dehydrogenases which seem to be specific for the D-isomer of their substrate have been shown to be functionally and structurally related. PHGDH catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor. Overexpression of PHGDH may cause certain breast cancers. Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency which is characterized by congenital microcephaly, psychomotor retardation, and seizures.

References

1. Pind S. et al., 2002, J Biol Chem. 277 (9): 7136-43.
2. Du H. et al., 2010, Reproduction. 139 (1): 237-45.
3. Possemato R. et al., 2011, Nature. 476 (7360): 346-50.