Rat PGC / pepsinogen C Protein (His Tag)

Progastricsin (pepsinogen C) Protein Datasheet

PGC / pepsinogen C Protein Product Information

• Synonym: PG1, Pg-1, Upg1
• Protein Construction: A DNA sequence encoding the rat PGC (NP_579818.1) (Met 1-Val 392) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

PGC / pepsinogen C Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 17
• Molecular Mass: The recombinant rat PGC comprises 387 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the ratPGC is approximately 43kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mMTris, 150mMNaCl, pH7.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PGC / pepsinogen C protein

PGC / pepsinogen C Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PGC / pepsinogen C Protein Description

Pepsinogen C, also known as PGC, is an aspartic proteinase that belongs to the peptidase family A1. Pepsinogen C is synthesized in the gastric mucosa as inactive precursors, known as zymogens. Pepsinogen C contains a prosegment that serves to stabilize the inactive form and prevent entry of the substrate to the active site. Upon ingestion of food, each of the zymogens is released into the gastric lumen and undergoes conversion into active enzyme in the acidic gastric juice. This activation reaction is nitiated by the disruption of electrostatic interactions between the prosegment and the active enzyme moiety at low pH values. Pepsinogen C has been found expressed in all regions of the stomach mucosa and also in the proximal duodenal mucosa. In stomach cancer tissues and cancer cell lines, the expressions of the pepsinogen genes were decreased or lost, in good accordance with their pepsinogen productions. No gross structural changes of the pepsinogen genes were observed in these cancers, but the methylation patterns of the pepsinogen genes were found to be altered in different ways in different cancers. Serum levels of Pepsinogen C are used as a biomarker for certain gastric diseases including Helicobacter pylori related gastritis.

References

1. Richter C. et al., 1998, Biochem J. 1 (335): 481-90.
2. Westerveld BD. et al., 1987, Gastroenterology. 93 (4): 774-8.
3. Ichinose M. et al., 1991, Jpn J Cancer Res. 82 (6): 686-92.