Mouse SERPINF1 Gene cDNA Clone / ORF Clone

SERPINF1 cDNA Clone Datasheet

SERPINF1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011340.3
• cDNA Size: 1254
• Gene Synonym: Pedf; Sdf3; EPC-1; Pedfl; AI195227; Serpinf1
• Source: Mouse

SERPINF1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus serine (or cysteine) peptidase inhibitor, clade F, member 1 DNA.
• Vector: Vector pMD18-T Simple
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 6 G/A not causing the amino acid variation.

SERPINF1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

SERPINF1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

SERPINF1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGGCCC TGGTGCTACT CCTCTGGACT GGAGCCCTGC TCGGGCACGG CAGCAGCCAG
61       AACGTCCCCA GCAGCTCTGA GGGCTCCCCA GTCCCGGACA GCACGGGCGA GCCCGTGGAG
121      GAGGAGGACC CCTTCTTCAA GGTCCCTGTG AACAAGCTGG CAGCAGCTGT CTCCAACTTC
181      GGCTACGATC TGTACCGCCT GAGATCCAGT GCCAGCCCAA CGGGCAACGT CCTGCTGTCT
241      CCACTCAGCG TGGCCACGGC CCTCTCTGCC CTTTCTCTGG GAGCTGAACA TCGAACAGAG
301      TCTGTCATTC ACCGGGCTCT CTACTACGAC CTGATCACCA ACCCTGACAT CCACAGCACC
361      TACAAGGAGC TCCTTGCCTC TGTTACTGCC CCTGAGAAGA ACCTCAAGAG TGCTTCCAGA
421      ATTGTGTTTG AGAGGAAACT TCGAGTCAAA TCCAGCTTTG TTGCCCCTCT GGAGAAGTCC
481      TATGGGACCA GGCCCCGGAT CCTCACGGGC AACCCTCGAG TAGACCTTCA GGAGATTAAC
541      AACTGGGTGC AGGCCCAGAT GAAAGGGAAG ATTGCCCGGT CCACGAGGGA AATGCCCAGT
601      GCCCTCAGCA TCCTTCTCCT TGGCGTGGCT TACTTCAAGG GGCAGTGGGT AACCAAGTTT
661      GACTCGAGAA AGACGACCCT CCAGGATTTT CATTTGGACG AGGACAGGAC CGTGAGAGTC
721      CCCATGATGT CAGATCCTAA GGCCATCTTA CGATACGGCT TGGACTCTGA TCTCAACTGC
781      AAGATTGCCC AGCTGCCCTT GACAGGAAGT ATGAGCATCA TCTTCTTCCT GCCCCTGACC
841      GTGACCCAGA ACTTGACCAT GATAGAAGAG AGCCTCACCT CTGAGTTCAT TCATGACATC
901      GACCGAGAAC TGAAGACTAT CCAAGCTGTG CTGACTGTCC CCAAGCTGAA GCTGAGCTTC
961      GAAGGCGAAC TTACCAAGTC TCTGCAGGAC ATGAAGCTAC AGTCGTTGTT TGAATCACCC
1021     GACTTCAGCA AGATTACTGG CAAACCCGTG AAGCTCACCC AAGTGGAACA CAGGGCTGCT
1081     TTCGAGTGGA ATGAAGAGGG GGCAGGAAGC AGCCCCAGCC CAGGCCTCCA GCCCGTCCGC
1141     CTCACCTTCC CGCTAGACTA TCACCTTAAC CAACCTTTCC TCTTTGTTCT GAGGGACACG
1201     GACACGGGGG CCCTCCTCTT CATAGGCAGA ATCCTGGACC CCAGTAGTAC TTAA

 

Quality Control:cDNA sequencing results is available upon request.