Anti-PEDF Antibody

Cat: 11104-RP02
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Anti-PEDF Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-PEDF Antibody
Validated applications
Species reactivity
Reacts with: Human
Human PEDF
Recombinant Human SerpinF1 protein (Catalog#11104-H08H)
Produced in rabbits immunized with purified, recombinant Human SerpinF1 (rh SerpinF1; Catalog#11104-H08H; NP_002606.3; Met 1-Pro 418). SerpinF1 specific IgG was purified by human SerpinF1 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PEDF Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PEDF Antibody (Rabbit Polyclonal antibody) Images
Human PEDF Immunoprecipitation(IP) 8054

SerpinF1 was immunoprecipitated using:

Lane A:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-SerpinF1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-SerpinF1 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 46 kDa

Observed band size: 46 kDa

Human PEDF Western blot (WB) 13344

Anti-SerpinF1 rabbit polyclonal antibody at 1:500 dilution

Lane A: K562 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:46 kDa

Observed band size:46 kDa

(We are unsure as to the identity of these extra bands.)

Anti-PEDF Antibody Alternative Names
Anti-EPC-1 Antibody;Anti-OI12 Antibody;Anti-OI6 Antibody;Anti-PEDF Antibody;Anti-PIG35 Antibody
PEDF Background Information

Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.

Full Name
serpin peptidase inhibitor, clade F,member 1
  • Ren, JG. et al., 2005, Med Hypotheses. 64 (1): 74-8.
  • Filleur, S. et al., 2009. J Cell Biochem. 106 (5): 769-75.
  • Kawaguchi, T. et al., 2010, Curr Mol Med. 10 (3): 302-11.
  • Yamagishi, SI. et al., 2010, Curr Mol Med. 10 (3): 284-91.
  • Nakamura, T. et al., 2010, Curr Mol Med. 10 (3): 312-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PEDF Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 80481-T60
    Anti-PEDF Antibody Rabbit Polyclonal ELISA 80481-T16
    Anti-PEDF Antibody Rabbit Polyclonal WB,ELISA,IHC-P 80481-T56
    Anti-PEDF Antibody Rabbit Monoclonal ELISA 80481-R061
    Anti-PEDF Antibody Rabbit Polyclonal WB,IHC-P,IP 80481-T44
    Anti-PEDF Antibody Rabbit Polyclonal ELISA 11104-T16
    Anti-PEDF Antibody Rabbit Polyclonal ELISA 11104-RP01
    Anti-PEDF Antibody Rabbit Monoclonal ELISA 11104-R013
    Anti-PEDF Antibody Rabbit Monoclonal WB 11104-R033
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