SerpinF1 / PEDF Antibody (Antigen Affinity Purified)

Pigment Epithelium-Derived Factor Antibody (Antigen Affinity Purified)

SerpinF1 / PEDF Antibody Product Information

• Immunogen: Recombinant human SerpinF1 protein ( Catalog#11104-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human SerpinF1 ( rh SerpinF1 ; Catalog#11104-H08H ; NP_002606.3 ; Met 1 - Pro 418 ). SerpinF1 specific IgG was purified by human SerpinF1 affinity chromatography.

SerpinF1 / PEDF Antibody Usage Guide

• Specificity: Human SerpinF1 / PEDF
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human SERPINF1 in WB. Using a DAB detection system, the detection limit for human SERPINF1 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.5 - 1.0 μg/mL with the appropriate secondary reagents to detect human SERPINF1. The detection limit for human SERPINF1 is 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

SerpinF1 / PEDF Antibody Background

Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.

References

1. Ren, JG. et al., 2005, Med Hypotheses. 64 (1): 74-8.
2. Filleur, S. et al., 2009. J Cell Biochem. 106 (5): 769-75.
3. Kawaguchi, T. et al., 2010, Curr Mol Med. 10 (3): 302-11.
4. Yamagishi, SI. et al., 2010, Curr Mol Med. 10 (3): 284-91.
5. Nakamura, T. et al., 2010, Curr Mol Med. 10 (3): 312-6.