SerpinF1 / SERPINF1 / PEDF Protein (His Tag)

Pigment Epithelium-Derived Factor ( SERPINF1 / PEDF ) Protein

SERPINF1 / PEDF Protein Product Information

• Synonym: EPC-1, PEDF, PIG35, SERPINF1
• Protein Construction: A DNA sequence encoding the human SERPINF1 ( NP_002606.3 ) ( Met 1- Pro 418 ) was fused with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

SERPINF1 / PEDF Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGEV
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 20
• Molecular Mass: The secreted recombinant human SERPINF1 consists of 410 amino acids with the predicted molecular mass of 45.8 kDa as estimated in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
• SDS-PAGE: SERPINF1 protein

SERPINF1 / PEDF Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SERPINF1 / PEDF Protein Description

Pigment epithelium-derived factor, also known as PEDF, Serpin F1, and SERPINF1, is a multiple functional protein which has both anti-angiogenic activity and neurotrophic activity at the same time. PEDF is a secreted glycoprotein that belongs to the noninhibitory serpin. It has an alpha/beta core serine-protease inhibitor domain, three major beta-sheets, and ten alpha-helices. PEDF does not inhibit either serine or cysteine proteinases. PEDF exerts diverse physiological activities including anti-angiogenesis, anti-vasopermeability, anti-tumor, and neurotrophic activities. PEDF acts via multiple high affinity ligands and cell receptors. It has been described as a natural angiogenesis inhibitor with neurotrophic and immune-modulation properties. PEDF induces macrophages apoptosis and necrosis through the activation of peroxisome proliferator-activated receptor-gamma by which PEDF could modulate inflammatory reactions in septic shock. It balances angiogenesis in the eye and blocks tumor progression.

References

1. Ren, JG. et al., 2005, Med Hypotheses. 64 (1): 74-8.
2. Filleur, S. et al., 2009. J Cell Biochem. 106 (5): 769-75.
3. Kawaguchi, T. et al., 2010, Curr Mol Med. 10 (3): 302-11.
4. Yamagishi, SI. et al., 2010, Curr Mol Med. 10 (3): 284-91.
5. Nakamura, T. et al., 2010, Curr Mol Med. 10 (3): 312-6.