Rat PEAR1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80245-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PEAR1 cDNA Clone Datasheet

PEAR1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001134959.1
cDNA Size:	3102
Gene Synonym:	MGC188434; RGD1305653; Pear1
Source:	Rat

PEAR1 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus platelet endothelial aggregation receptor 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 200 T/C and 2050 C/T resulting in the amino acid Met substitution by Thr, Arg substitution by Trp.

PEAR1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PEAR1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PEAR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCACTCT GTCCCCTCCT CCTCCTGGCC CTAGGCCTGC GTCTGGCTGG AACACTCAAC
61       TCCAATGATC CCAATGTCTG TACCTTCTGG GAAAGCTTCA CCACGACCAC TAAGGAGTCC
121      CACCTTCGCC CCTTCAGCCT GCCCCCAGCC GAGTCCTGCG ACAGGCCCTG GGAAGACCCC
181      CACACCTGCG CTCAGCCTAC GGTTGTCTAC CGGACTGTGT ACCGTCAGGT GGTGAAGATG
241      GACTCCCGCC CACGCCTGCA GTGCTGTGGG GGTTACTACG AGAGCAGTGG AGCCTGTGTC
301      CCACTCTGTG CCCAGGAGTG TGTCCACGGT CGCTGTGTGG CTCCTAATCG GTGCCAGTGT
361      GCACCAGGCT GGCGGGGTGA CGACTGTTCC AGTGAGTGTG CTCCTGGAAT GTGGGGACCA
421      CAGTGTGACA GGCTCTGCCT CTGTGGCAAC AGCAGTTCCT GTGATCCCAG GAGTGGGGTG
481      TGTTTTTGCC CCTCTGGCCT GCAGCCCCCC GACTGCCTTC AGCCTTGCCC CGATGGCCAC
541      TATGGTCCTG CCTGCCAGTT TGATTGCCAT TGCTATGGGG CATCCTGTGA CCCCCGGGAT
601      GGAGCCTGCT TCTGCCCCCC AGGGAGAACA GGACCCAGCT GTAATGTGCC CTGTTCACAG
661      GGCACTGATG GCTTCTTCTG CCCCAGAACT TATCCTTGCC AAAATGGAGG TGTTCCTCAG
721      GGCTCTCAAG GCTCCTGCAG CTGCCCACCG GGCTGGATGG GTGTCATCTG TTCCCTGCCA
781      TGCCCAGAGG GTTTCCACGG ACCCAACTGT ACTCAGGAAT GTCGTTGCCA CAATGGTGGC
841      CTTTGTGACA GGTTTACTGG GCAGTGCCAC TGTGCTCCTG GCTATATCGG GGATCGGTGC
901      CGTGAAGAGT GCCCTGTGGG CCGCTTCGGT CAAGACTGTG CTGAGACCTG TGACTGTGCT
961      CCTGGCGCTC GTTGCTTTCC TGCCAATGGC GCGTGTCTGT GCGAACATGG CTTCACAGGC
1021     GACCGCTGCA CTGAGCGACT CTGTCCAGAT GGCCGCTATG GTCTGAGCTG CCAAGATCCC
1081     TGCACCTGCG ACCCAGAACA CAGTCTCAGC TGCCACCCAA TGCACGGCGA GTGCTCCTGC
1141     CAGCCAGGTT GGGCGGGCCT CCACTGCAAC GAGAGCTGCC CTCAGGACAC GCACGGAGCC
1201     GGTTGCCAGG AGCACTGCCT CTGTCTGCAC GGCGGTGTTT GCCTCGCCGA CAGCGGCCTC
1261     TGCCGGTGTG CACCTGGCTA CACGGGACCT CACTGCGCTA ATCTTTGTCC ACCTAACACT
1321     TATGGGATCA ACTGTTCCTC CCACTGCTCC TGTGAAAATG CCATTGCCTG CTCTCCTGTC
1381     GACGGCACGT GCATCTGCAA GGAAGGTTGG CAGCGTGGTA ACTGCTCTGT GCCCTGTCCC
1441     CCTGGCACCT GGGGCTTCAG TTGCAATGCC AGTTGCCAGT GTGCCCACGA GGGAGTCTGC
1501     AGCCCCCAAA CTGGAGCCTG TACTTGCACC CCTGGGTGGC GTGGGGTTCA CTGCCAACTT
1561     CCGTGCCCGA AGGGACAGTT TGGTGAAGGT TGTGCCAGTG TCTGTGACTG TGACCACTCC
1621     GATGGCTGTG ACCCTGTTCA TGGACACTGC CGATGTCAGG CTGGCTGGAT GGGCACACGT
1681     TGCCACCTGC CTTGCCCAGA GGGCTTTTGG GGAGCCAACT GCAGCAATGC CTGTACCTGC
1741     AAGAATGGTG GCACTTGTGT ACCTGAGAAC GGCAACTGTG TGTGCGCACC AGGGTTCAGA
1801     GGCCCCTCCT GCCAGAGGCC CTGCCCGCCT GGTCGCTATG GCAAACGCTG TGTGCCCTGC
1861     AAGTGCAACA ACCATTCTTC CTGCCACCCG TCGGATGGGA CCTGCTCCTG CCTGGCAGGC
1921     TGGACAGGCC CTGACTGCTC TGAATCATGT CCCCCAGGCC ACTGGGGACT CAAATGCTCC
1981     CAACCCTGCC AGTGTCATCA TGGTGCCACC TGCCACCCCC AGGATGGGAG CTGTGTCTGC
2041     ATCCCAGGCT GGACTGGACC CAACTGCTCG GAAGGCTGCC CATCAAGAAT GTTTGGTGTC
2101     AACTGCTCCC AGCTATGTCA GTGTGATCCT GGAGAGATGT GCCACCCAGA GACTGGGGCT
2161     TGCGTCTGTC CCCCAGGACA CAGTGGTGCG CACTGCAAAG TGGGCAGCCA GGAGTCCTTC
2221     ACCATAATGC CCACCTCTCC TGTGATCCAT AACTCACTGG GTGCCGTGAT TGGCATTGCA
2281     GTGCTGGGGA CCCTTGTGGT GGCCCTGGTA GCACTGTTTA TTGGCTACCG ACACTGGCAA
2341     AAGGGCAAGG AACATGAGCA CTTGGCAGTG GCTTACAGCA CTGGGCGACT GGATGGCTCC
2401     GATTACGTCA TGCCAGATGT CTCTCCGAGC TACAGTCACT ACTATTCCAA CCCTAGCTAC
2461     CACACACTGT CTCAGTGTTC TCCTAACCCT CCACCCCCTA ACAAGATTCC AGGCAGTCAG
2521     CTGTTTGTCA GCTCCCAGGC ATCTGAGCGG CCAAACAGAA ACCATGGGCG AGATAACCAC
2581     GCCACACTGC CCGCTGACTG GAAGCACCGA CGGGAGTCCC ATGACAGAGG CACCAGCCAC
2641     CTGGACCGAA GGTATAGCTG TAGCTATGGC CACAGGAATG GCCCGGGGCC ATTCTGTCAT
2701     AAAGGTCCCA TCTCTGAAGA AGGACTAGGG GCAAGCGTTA TGTCCCTGAG CAGTGAGAAC
2761     CCCTATGCGA CCATCCGAGA CCTGCCCGGC CTGCCTGGGG AACCCCGAGA AAGCAGCTAT
2821     GTGGAGATGA AAGGCCCTCC ATCAGTGTCT CCCCCCAGGC AGCCTCTTCA TCTCCGGGAC
2881     AGGCAGCAGC AGCAACTGCA GTCTCAGAGA GACAGCGGCA CCTATGAGCA GCCCACTCCC
2941     TTGAGCCGTA ATGAAGAGTC TGTGGGCTCC ATGCCCCCTC TTCCTCCGGG CCTGCCACCC
3001     GGCCACTATG ACTCGCCCAA AAACAGCCAC ATCCCTGGAC ACTATGACTT GCCTCCAGTA
3061     CGGCATCCTC CATCACCTCC ATCCCGGCGC CAGGACCGCT GA

Quality Control:cDNA sequencing results is available upon request.