Mouse PDPK1 Gene cDNA Clone / ORF Clone

PDPK1 cDNA Clone Datasheet

PDPK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011062.4
• cDNA Size: 1680
• Gene Synonym: Pdk1; Pdpk1
• Source: Mouse

PDPK1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus 3-phosphoinositide dependent protein kinase 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PDPK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDPK1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDPK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCTGTGCCCA TTCAGTCCAG TGTGGTGCTA
61       TGTTCCTGCC CATCCCCATC AATGGTGAGG TCCCAGACTG AGCCCGGTTC GTCCCCTGGC
121      ATTCCTAGTG GTGTTAGCAG GCAGGGATCC ACCATGGATG GCACCACAGC TGAAGCCCGA
181      CCAAGCACCA ACCCCTTGCA GCAGCACCCT GCCCAGCTGC CACCACAGCC TCGCAAGAAA
241      CGCCCTGAAG ACTTCAAGTT TGGGAAAATT CTTGGCGAGG GCTCTTTTTC AACAGTTGTT
301      CTGGCCCGAG AACTGGCCAC TTCCAGAGAA TATGCTATTA AAATTCTGGA GAAACGTCAT
361      ATTATAAAAG AAAACAAAGT TCCGTATGTA ACTAGAGAGA GAGATGTGAT GTCACGCCTG
421      GATCACCCCT TCTTTGTGAA ACTTTATTTT ACATTTCAGG ACGACGAAAA GCTGTATTTT
481      GGCCTTAGTT ATGCCAAAAA TGGAGAGCTA CTTAAATACA TCCGCAAAAT TGGCTCATTT
541      GATGAGACCT GTACCCGGTT TTACACGGCT GAGATTGTGT CTGCTTTAGA GTACTTGCAT
601      GGCAAGGGCA TCATTCACAG AGACCTTAAA CCAGAAAACA TTTTGTTAAA TGAAGACATG
661      CACATCCAGA TCACAGATTT TGGAACAGCA AAAGTGTTAT CCCCAGAGAG CAAACAAGCC
721      AGGGCCAACT CATTTGTAGG AACAGCACAG TATGTTTCTC CAGAGCTGCT CACAGAGAAG
781      TCGGCGTGTA AAAGTTCAGA CCTTTGGGCC CTTGGATGTA TAATCTATCA GCTCGTGGCA
841      GGACTCCCAC CATTCAGAGC CGGGAATGAA TATCTTATAT TTCAGAAGAT CATTAAGCTG
901      GAATATCATT TCCCAGAAAA ATTCTTCCCT AAGGCTAGAG ATCTTGTGGA AAAACTCTTG
961      GTTTTAGATG CCACAAAGCG TTTAGGCTGT GAAGAGATGG AAGGGTACGG GCCTCTCAAA
1021     GCTCATCCAT TCTTTGAGAC CATCACTTGG GAGAATTTGC ACCAGCAGAC ACCTCCGAAG
1081     CTCACAGCTT ACCTACCAGC CATGTCAGAG GATGATGAAG ACTGCTATGG CAACTACGAC
1141     AATCTCCTGA GCCAGTTTGG CTTCATGCAG GTGTCATCCT CCTCCTCTTC CCACTCCCTG
1201     TCTACGGTGG AAACCAGCCT GCCCCAGAGG TCGGGCAGCA ACATAGAGCA GTACATCCAT
1261     GATTTGGACA CTAACTCTTT TGAACTAGAC TTACAGTTTT CAGAAGATGA AAAAAGGTTG
1321     TTATTGGAAA AGCAAGCCGG TGGAAACCCT TGGCACCAGT TTGTAGAAAA TAATCTAATA
1381     TTAAAAATGG GTCCAGTGGA TAAGCGAAAG GGTTTATTTG CAAGACGACG ACAGTTATTA
1441     CTCACAGAAG GGCCACATTT ATATTATGTT GATCCTGTCA ACAAGGTCTT GAAAGGTGAA
1501     ATCCCATGGT CACAAGAACT CCGACCAGAA GCCAAGAATT TTAAAACTTT CTTTGTCCAC
1561     ACGCCTAACA GGACGTACTA CCTGATGGAT CCAAGCGGGA ATGCTCACAA GTGGTGCAGA
1621     AAGATCCAGG AGGTTTGGAG GCAGCAGTAC CAGAGCAATC CAGATGCTGC TGTGCAGTGA

 

Quality Control:cDNA sequencing results is available upon request.