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Human PDPK1 Gene cDNA Clone (full-length ORF Clone)
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Human PDPK1 Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10681-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services
Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_002613.3

cDNA Size:   1671

Synonym:  PDK1; PRO0461; MGC20087; MGC35290; PDPK1

Source:  Human. cDNA Description:  ORF Clone of Homo sapiens 3-phosphoinositide dependent protein kinase-1 DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG


Sequence Description:  Identical with the Gene Bank Ref. ID sequence. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15–30 min .
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGCCAGGA CCACCAGCCA GCTGTATGAC GCCGTGCCCA TCCAGTCCAG CGTGGTGTTA
61 TGTTCCTGCC CATCCCCATC AATGGTGAGG ACCCAGACTG AGTCCAGCAC GCCCCCTGGC
121 ATTCCTGGTG GCAGCAGGCA GGGCCCCGCC ATGGACGGCA CTGCAGCCGA GCCTCGGCCC
181 GGCGCCGGCT CCCTGCAGCA TGCCCAGCCT CCGCCGCAGC CTCGGAAGAA GCGGCCTGAG
241 GACTTCAAGT TTGGGAAAAT CCTTGGGGAA GGCTCTTTTT CCACGGTTGT CCTGGCTCGA
301 GAACTGGCAA CCTCCAGAGA ATATGCGATT AAAATTCTGG AGAAGCGACA TATCATAAAA
361 GAGAACAAGG TCCCCTATGT AACCAGAGAG CGGGATGTCA TGTCGCGCCT GGATCACCCC
421 TTCTTTGTTA AGCTTTACTT CACATTTCAG GACGACGAGA AGCTGTATTT CGGCCTTAGT
481 TATGCCAAAA ATGGAGAACT ACTTAAATAT ATTCGCAAAA TCGGTTCATT CGATGAGACC
541 TGTACCCGAT TTTACACGGC TGAGATTGTG TCTGCTTTAG AGTACTTGCA CGGCAAGGGC
601 ATCATTCACA GGGACCTTAA ACCGGAAAAC ATTTTGTTAA ATGAAGATAT GCACATCCAG
661 ATCACAGATT TTGGAACAGC AAAAGTCTTA TCCCCAGAGA GCAAACAAGC CAGGGCCAAC
721 TCATTCGTGG GAACAGCGCA GTACGTTTCT CCAGAGCTGC TCACGGAGAA GTCCGCCTGT
781 AAGAGTTCAG ACCTTTGGGC TCTTGGATGC ATAATATACC AGCTTGTGGC AGGACTCCCA
841 CCATTCCGAG CTGGAAACGA GTATCTTATA TTTCAGAAGA TCATTAAGTT GGAATATGAC
901 TTTCCAGAAA AATTCTTCCC TAAGGCAAGA GACCTCGTGG AGAAACTTTT GGTTTTAGAT
961 GCCACAAAGC GGTTAGGCTG TGAGGAAATG GAAGGATACG GACCTCTTAA AGCACACCCG
1021 TTCTTCGAGT CCGTCACGTG GGAGAACCTG CACCAGCAGA CGCCTCCGAA GCTCACCGCT
1081 TACCTGCCGG CTATGTCGGA AGACGACGAG GACTGCTATG GCAATTATGA CAATCTCCTG
1141 AGCCAGTTTG GCTGCATGCA GGTGTCTTCG TCCTCCTCCT CACACTCCCT GTCAGCCTCC
1201 GACACGGGCC TGCCCCAGAG GTCAGGCAGC AACATAGAGC AGTACATTCA CGATCTGGAC
1261 TCGAACTCCT TTGAACTGGA CTTACAGTTT TCCGAAGATG AGAAGAGGTT GTTGTTGGAG
1321 AAGCAGGCTG GCGGAAACCC TTGGCACCAG TTTGTAGAAA ATAATTTAAT ACTAAAGATG
1381 GGCCCAGTGG ATAAGCGGAA GGGTTTATTT GCAAGACGAC GACAGCTGTT GCTCACAGAA
1441 GGACCACATT TATATTATGT GGATCCTGTC AACAAAGTTC TGAAAGGTGA AATTCCTTGG
1501 TCACAAGAAC TTCGACCAGA GGCCAAGAAT TTTAAAACTT TCTTTGTCCA CACGCCTAAC
1561 AGGACGTATT ATCTGATGGA CCCCAGCGGG AACGCACACA AGTGGTGCAG GAAGATCCAG
1621 GAGGTTTGGA GGCAGCGATA CCAGAGCCAC CCGGACGCCG CTGTGCAGTG A

Quality Control:   cDNA sequencing results is available upon request.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"