Mouse PDK4 Gene cDNA Clone / ORF Clone

PDK4 cDNA Clone Datasheet

PDK4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013743.2
• cDNA Size: 1239
• Gene Synonym: AV005916; Pdk4
• Source: Mouse

PDK4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence.

PDK4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDK4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDK4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGGCAG CCCGCTTCGT GATGCGCAGC GCCAGCTCGC TGAGCAGCGC CAGCCTGGTC
61       CCCAGGGAGG TCGAGCTGTT CTCCCGCTAC AGCCCGTCCC CGCTGTCCAT GAAGCAGCTG
121      CTGGACTTTG GTTCAGAAAA TGCCTGTGAA AGAACGTCCT TTGCTTTTCT GCGGCAAGAG
181      CTGCCCGTCC GCCTGGCCAA TATCCTGAAG GAGATTGACA TCCTGCCTGA CCGCTTAGTG
241      AACACTCCTT CGGTGCAGCT GGTGAAGAGC TGGTATATCC AGAGCCTGAT GGATTTGGTG
301      GAGTTCCATG AGAAGAGCCC AGAAGACCAG AAAGCCCTGT CAGAGTTTGT AGACACGCTG
361      GTCAAAGTTC GAAACAGACA TCATAATGTG GTCCCTACAA TGGCTCAAGG CATCCTGGAG
421      TATAAAGACA CCTGCACAGT GGACCCCGTT ACCAATCAAA ATCTTCAGTA TTTTTTAGAC
481      CGGTTTTACA TGAACCGCAT TTCTACTCGG ATGCTCATGA ATCAGCACAT CCTCATATTC
541      AGTGACTCAA AGACGGGAAA CCCAAGCCAC ATTGGAAGTA TCGACCCAAA CTGTGATGTG
601      GTAGCAGTAG TCCAAGATGC CTTTGAGTGT GCAAAGATGC TCTGCGACCA GTATTATCTA
661      ACATCGCCAG AATTAAACCT CACACAAGTC AATGGAAAAT TTCCAGGCCA ACCAATCCAC
721      ATTGTGTACG TTCCTTCACA CCTTCACCAC ATGCTCTTCG AACTCTTCAA GAATGCCATG
781      AGGGCCACGG TCGAGCATCA AGAAAACCGT CCTTCCTTGA CCCCAGTAGA GGCCACTGTC
841      GTCTTGGGAA AAGAAGACCT TACAATCAAG ATTTCTGACC GAGGAGGCGG TGTTCCTCTG
901      AGGATTACTG ACCGCCTCTT TAGTTACACG TACTCCACTG CTCCAACACC TGTGATGGAC
961      AATTCCCGGA ATGCCCCTTT GGCTGGTTTT GGTTATGGCT TGCCAATTTC TCGTCTCTAC
1021     GCCAAGTATT TTCAAGGAGA TCTGAATCTC TACTCTATGT CAGGTTATGG GACAGACGCT
1081     ATCATCTACT TAAAGGCTTT ATCTTCTGAG TCTGTAGAAA AGCTCCCAGT CTTTAACAAG
1141     TCAGCCTTCA AACATTATCA GATGAGCTCC GAAGCTGATG ACTGGTGTAT CCCAAGCAGG
1201     GAACCGAAGA ACCTGGCGAA GGAGAAGCTG GCAGTGTGA

 

Quality Control:cDNA sequencing results is available upon request.