Human PDK2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12305-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PDK2 cDNA Clone Datasheet

PDK2 cDNA Clone Product Information

Gene Bank Ref.ID:	BC005811
cDNA Size:	1224
Gene Synonym:	PDHK2; PDK2
Source:	Human

PDK2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens pyruvate dehydrogenase kinase, isozyme 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PDK2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDK2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDK2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGCTGGG TGTGGGCGCT GCTGAAGAAT GCGTCCCTGG CAGGGGCGCC CAAGTACATA
61       GAGCACTTCA GCAAGTTCTC CCCGTCCCCG CTGTCCATGA AGCAGTTTCT GGACTTCGGA
121      TCCAGCAATG CCTGTGAGAA AACCTCCTTC ACCTTCCTCA GGCAGGAGCT GCCTGTGCGC
181      CTGGCCAACA TCATGAAAGA GATCAACCTG CTTCCCGACC GAGTGCTGAG CACACCCTCC
241      GTGCAGCTGG TGCAGAGCTG GTATGTCCAG AGCCTCCTGG ACATCATGGA GTTCCTGGAC
301      AAGGATCCCG AGGACCATCG CACCCTGAGC CAGTTCACTG ACGCCCTGGT CACCATCCGG
361      AACCGGCACA ACGACGTGGT GCCCACCATG GCACAAGGCG TGCTTGAGTA CAAGGACACC
421      TACGGCGATG ACCCCGTCTC CAACCAGAAC ATCCAGTACT TCCTGGACCG CTTCTACCTC
481      AGCCGCATCT CCATCCGCAT GCTCATCAAC CAGCACACCC TCATCTTTGA TGGCAGCACC
541      AACCCAGCCC ATCCCAAACA CATCGGCAGC ATCGACCCCA ACTGCAACGT CTCTGAGGTG
601      GTCAAAGATG CCTACGACAT GGCTAAGCTC CTGTGTGACA AGTATTACAT GGCCTCACCT
661      GACCTGGAGA TCCAGGAGAT CAATGCAGCC AACTCCAAAC AGCCGATTCA CATGGTCTAC
721      GTCCCCTCCC ACCTCTACCA CATGCTCTTT GAGCTCTTCA AGAATGCCAT GAGGGCGACT
781      GTGGAAAGCC ATGAGTCCAG CCTCATTCTC CCACCCATCA AGGTCATGGT GGCCTTGGGT
841      GAGGAAGATC TGTCCATCAA GATGAGTGAC CGAGGTGGGG GTGTTCCCTT GAGGAAGATT
901      GAGCGACTCT TCAGCTACAT GTACTCCACA GCACCCACCC CCCAGCCTGG CACCGGGGGA
961      ACGCCGCTGG CTGGCTTTGG TTATGGGCTC CCCATTTCCC GCCTCTACGC CAAGTACTTC
1021     CAGGGAGACC TGCAGCTCTT CTCCATGGAA GGCTTTGGGA CCGATGCTGT CATCTATCTC
1081     AAGGCCCTGT CCACGGACTC GGTGGAGCGC CTGCCTGTCT ACAACAAGTC AGCCTGGCGC
1141     CACTACCAGA CCATCCAGGA GGCCGGCGAC TGGTGTGTGC CCAGCACGGA GCCCAAGAAC
1201     ACGTCCACGT ACCGCGTCAG CTAA

Quality Control:cDNA sequencing results is available upon request.