Human PDK1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12312-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PDK1 cDNA Clone Datasheet

PDK1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC039158
cDNA Size:	1311
Gene Synonym:	PDK1
Source:	Human

PDK1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens pyruvate dehydrogenase kinase, isozyme 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PDK1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDK1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGCTGG CGCGGCTGCT TCGCGGAGCC GCCTTGGCCG GCCCGGGCCC GGGGCTGCGC
61       GCCGCCGGCT TCAGCCGCAG CTTCAGCTCG GACTCGGGCT CCAGCCCGGC GTCCGAGCGC
121      GGCGTTCCGG GCCAGGTGGA CTTCTACGCG CGCTTCTCGC CGTCCCCGCT CTCCATGAAG
181      CAGTTCCTGG ACTTCGGATC AGTGAATGCT TGTGAAAAGA CCTCATTTAT GTTTCTGCGG
241      CAAGAGTTGC CTGTCAGACT GGCAAATATA ATGAAAGAAA TAAGTCTCCT TCCAGATAAT
301      CTTCTCAGGA CACCATCCGT TCAATTGGTA CAAAGCTGGT ATATCCAGAG TCTTCAGGAG
361      CTTCTTGATT TTAAGGACAA AAGTGCTGAG GATGCTAAAG CTATTTATGA CTTTACAGAT
421      ACTGTGATAC GGATCAGAAA CCGACACAAT GATGTCATTC CCACAATGGC CCAGGGTGTG
481      ATTGAATACA AGGAGAGCTT TGGGGTGGAT CCTGTCACCA GCCAGAATGT TCAGTACTTT
541      TTGGATCGAT TCTACATGAG TCGCATTTCA ATTAGAATGT TACTCAATCA GCACTCTTTA
601      TTGTTTGGTG GAAAAGGCAA AGGAAGTCCA TCTCATCGAA AACACATTGG AAGCATAAAT
661      CCAAACTGCA ATGTACTTGA AGTTATTAAA GATGGCTATG AAAATGCTAG GCGTCTGTGT
721      GATTTGTATT ATATTAACTC TCCCGAACTA GAACTTGAAG AACTAAATGC AAAATCACCA
781      GGACAGCCAA TACAAGTGGT TTATGTACCA TCCCATCTCT ATCACATGGT GTTTGAACTT
841      TTCAAGAATG CAATGAGAGC CACTATGGAA CACCATGCCA ACAGAGGTGT TTACCCCCCT
901      ATTCAAGTTC ATGTCACGCT GGGTAATGAG GATTTGACTG TGAAGATGAG TGACCGAGGA
961      GGTGGCGTTC CTTTGAGGAA AATTGACAGA CTTTTCAACT ACATGTATTC AACTGCACCA
1021     AGACCTCGTG TTGAGACCTC CCGCGCAGTG CCTCTGGCTG GTTTTGGTTA TGGATTGCCC
1081     ATATCACGTC TTTACGCACA ATACTTCCAA GGAGACCTGA AGCTGTATTC CCTAGAGGGT
1141     TACGGGACAG ATGCAGTTAT CTACATTAAG GCTCTGTCAA CAGACTCAAT AGAAAGACTC
1201     CCAGTGTATA ACAAAGCTGC CTGGAAGCAT TACAACACCA ACCACGAGGC TGATGACTGG
1261     TGCGTCCCCA GCAGAGAACC CAAAGACATG ACGACGTTCC GCAGTGCCTA G

Quality Control:cDNA sequencing results is available upon request.