Cynomolgus monkey PDGFRB Gene cDNA Clone / ORF Clone

PDGFRB cDNA Clone Datasheet

PDGFRB cDNA Clone Product Information

• cDNA Size: 3321
• Gene Synonym: PDGFRB
• Source: Cynomolgus monkey

PDGFRB cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) platelet-derived growth factor receptor, beta polypeptide DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 273 T/C, 1032 T/A, 1596 A/G, 2211 T/C, 2253 C/T and 2664 C/T not causing the amino acid variation.
  Please check the sequence information before order.

PDGFRB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDGFRB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDGFRB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCGGCTTC CGGGTGCGAT GCCAGCTCTG GCCCTCAAAG GCCAGCTGCT GTTGCTGCCC
61       CTGCTGTTAC TGCTGGAACC GCAGGTCTCT CAGGGCCTGG TCATCACACC CCCGGGGCCA
121      GAGCTTATCC TCAATGTCTC CAGCACCTTT GTTCTGACCT GCTCGGGTTC AGCTCCCGTG
181      GTGTGGGAGC GGATGTCCCA GGAGCTCCCA CAGGAAATGG CCAAGGCCCA GGACAATACC
241      TTCTCCAGTG TGCTGACACT GACCAACCTC ACTGGGCTAG ACACGGGAGA ATACTTTTGC
301      ACCTACAATG ACTCCCGTGG ACTGGAGCCT GATGAGCGGA AACGGCTCTA CATCTTTGTG
361      CCAGATCCCA CCATGGGCTT CCTCCCTAAT GACGCCGAGG AACTATTCAT CTTTCTTACG
421      GAAATAACTG AGATCACCAT TCCATGCCGA GTAACAGACC CACAACTGGT GGTGACACTG
481      CACGAGAAGA AAGGGGACAT TGCACTGCCT GTCCCCTACG ATCACCAGCG TGGCTTTTCT
541      GGTATCTTTG AGGACAGAAG CTACATCTGC AAAACCACCA TTGGGGACAG GGAGGTGGAT
601      TCCGACGCCT ACTATGTCTA CAGACTCCAG GTGTCATCCA TCAACGTCTC CGTGAATGCA
661      GTGCAGACGG TGGTCCGCCA GGGTGAGAAC ATTACCCTCA TGTGCATTGT GATCGGGAAT
721      GAGGTGGTCA ACTTCGAGTG GATGTACCCC CGCAAAGAAA GTGGGCGGCT GGTGGAGCCG
781      GTGACCGACT TCCTCTTGGA TATGCCTTAC CACATCCGCT CCATCCTGCA CATCCCCAGT
841      GCCGAGTTAG AAGACTCGGG GACCTACACC TGCAATGTGA CAGAGAGTGT GAATGACCAT
901      CAGGATGAAA AGGCCATCAA CATCACTGTG GTTGAGAGTG GCTACGTGCG GCTCCTGGGA
961      GAGGTGGGAG CACTACAATT TGCTGAGCTG CACCGGAGCC GGACACTGCA GGTAGTGTTC
1021     GAGGCCTACC CACCGCCCAC CGTCCTGTGG TTCAAAGACA ACCGCACCTT GGGCGACTCC
1081     AGCGCAGGCG AGATCGCCCT GTCCACGCGC AACGTGTCAG AGACCAGGTA TGTGTCAGAG
1141     CTGACACTCG TTCGGGTGAA GGTGGCAGAG GCTGGCCACT ACACCATGCG GGCCTTCCAT
1201     GAGGACGCTG AGGTCCAGCT CTCCTTCCAG CTACAGATCA ATGTCCCTGT CCGCGTGCTG
1261     GAGCTAAGTG AGAGCCACCC AGACAGCGGG GAACAGACAG TCCGCTGTCG TGGCCGGGGC
1321     ATGCCCCAGC CGAACATCAT CTGGTCTGCC TGCAGAGACC TCAAAAGGTG TCCACGCGAG
1381     CTGCCGCCCA TGCTGCTGGG GAACAGTTCT GAAGAGGAGA GCCAGCTGGA GACGAACGTG
1441     ACATACTGGG AGGAGGAGCA GGAGTTTGAG GTGGTGAGCA CACTGCGTCT GCAGCACGTG
1501     GATCGGCCAC TGTCGGTGCG CTGCACGCTG CGCAACGCTG TGGGCCAGGA CATGCAGGAG
1561     GTCATCGTGG TGCCACACTC TTTGCCCTTC AAGGCGGTGG TGATCTCAGC CATCCTGGCC
1621     CTGGTGGTCC TCACCATCAT CTCCCTTATC ATCCTCATCA TGCTTTGGCA GAAGAAGCCA
1681     CGTTACGAGA TCCGATGGAA GGTGATTGAG TCTGTGAGCT CTGATGGCCA TGAGTACATC
1741     TACGTGGACC CCATGCAGCT GCCCTATGAC TCCACGTGGG AGCTGCCGCG GGACCAGCTT
1801     GTGCTGGGAC GCACCCTCGG CTCTGGGGCC TTTGGGCAGG TGGTGGAGGC CACGGCTCAT
1861     GGCCTGAGCC ATTCTCAGGC CACGATGAAA GTGGCCGTCA AGATGCTTAA ATCCACAGCC
1921     CGCAGCAGTG AGAAGCAAGC CCTCATGTCG GAGCTGAAGA TCATGAGTCA CCTTGGGCCC
1981     CACCTGAACG TGGTCAACCT GTTGGGGGCC TGCACCAAAG GAGGACCCAT CTATATCATC
2041     ACTGAGTACT GCCGCTACGG AGACCTGGTG GACTACCTGC ACCGCAACAA GCACACGTTC
2101     CTGCAGCACC ATTCCGACAA GCGCCGCCCG CCCAGCGCGG AGCTCTACAG CAATGCGCTG
2161     CCCGTTGGGC TCCCCCTGCC CAGCCACGTG TCCCTGACTG GGGAGAGCGA CGGTGGCTAC
2221     ATGGACATGA GCAAGGACGA GTCGGTGGAC TATGTGCCCA TGCTGGACAT GAAAGGAGAT
2281     GTCAAATATG CCGACATCGA GTCCTCCAAC TACATGGCCC CCTACGATAA CTACGTTCCC
2341     TCTGCCCCTG AGAGGACCTG TCGGGCAACT TTAATCAATG AGTCTCCGGT GCTAAGCTAC
2401     ATGGACCTTG TGGGCTTCAG CTACCAGGTG GCCAATGGCA TGGAGTTCCT GGCCTCCAAG
2461     AACTGCGTGC ACCGAGACCT GGCGGCCAGG AACGTGCTCA TCTGCGAGGG CAAGCTGGTC
2521     AAGATCTGTG ACTTTGGCCT GGCTCGAGAC ATCATGCGGG ATTCGAATTA CATCTCCAAA
2581     GGCAGTACCT TTTTGCCTTT GAAGTGGATG GCTCCAGAGA GCATCTTCAA CAGCCTCTAC
2641     ACCACCCTGA GCGACGTGTG GTCTTTCGGG ATCCTGCTCT GGGAGATCTT CACTTTGGGT
2701     GGCACCCCTT ACCCAGAGCT GCCCATGAAT GAGCAGTTCT ACAATGCCAT CAAACGGGGT
2761     TACCGCATGG CCCAGCCTGC CCACGCCTCC GACGAGATCT ATGAGATCAT GCAGAAGTGC
2821     TGGGAAGAGA AGTTTGAGAT TCGGCCCCCT TTCTCCCAGC TGGTGCTGCT TCTCGAGAGA
2881     CTGTTGGGCG AAGGTTACAA AAAGAAGTAC CAGCAGGTGG ATGAGGAGTT TCTGAGGAGT
2941     GACCACCCAG CCATCCTTCG GTCCCAGGCC CGCTTGCCTG GGTTCCATGG CCTCCGATCT
3001     CCCCTGGACA GCAGCTCCGT CCTCTATACC GCCGTGCAGC CCAATGAGGG TGACAACGAC
3061     TATATCATCC CCCTGCCTGA CCCCAAACCC GAGGTTGCTG ACGAGGGCCC ACTGGAGGGT
3121     TCCCCCAGCC TAGCCAGCTC CACCCTGAAT GAAGTCAACA CCTCCTCGAC CATCTCCTGT
3181     GACAGCCCTC TGGAGCCCCA GGAGGAACCA GAGCCAGAGC CCCAGCTTGA GCGCCAGGTG
3241     GAACCAGAGC CAGAGCTGGA ACAGCTGCCG GATTCAGGGT GCCCCGCGCC TCGAGCGGAA
3301     GCAGAGGACA GCTTCCTGTA G

 

Quality Control:cDNA sequencing results is available upon request.