Rat PDGFRB Gene cDNA Clone / ORF Clone

PDGFRB cDNA Clone Datasheet

PDGFRB cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031525.1
• cDNA Size: 3294
• Gene Synonym: Pdgfrb
• Source: Rat

PDGFRB cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus platelet derived growth factor receptor, beta polypeptide DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1851 C/G，1875 A/G，1951 T/C and 2400 A/G not causing the amino acid variation.

PDGFRB cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDGFRB cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDGFRB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGCTTC CAGAGGTGAT GCCAGCTTCA GTCCTCAGAG GCCAATTGTT GCTGTTCGTG
61       CTATTGCTCC TGGGACCACA GATCTCCCAA GGCCTAGTCA TCACGCCCCC TGGGCCAGAG
121      TTCGTCCTCA ACATTTCGAG CACCTTTGTT CTGACATGCT CAAGTTCAGC TCCAGTGATG
181      TGGGAACAGA TGTCCCAGGT GCCCTGGCAA GAAGCAGCCA TGAACCAGGA TGGCACCTTC
241      TCCAGTGTGC TGACACTGAC TAATGTCACT GGGGGAGACA CCGGAGAATA CTTTTGTGTC
301      TACAACAACT CACTGGGGCC AGAGCTCAGT GAGCGGAAGC GCATCTATAT CTTTGTGCCA
361      GATCCCACCA TGGGCTTCCT CCCGATGGAC TCTGAGGACC TATTCATTTT TGTCACGGAT
421      GTCACTGAGA CGACGATTCC ATGCCGAGTG ACAGACCCCC AGCTGGAGGT GACGCTGCAT
481      GAGAAGAAAG TGGATATCCC CCTACATGTA CCCTATGACC ACCAGCGAGG TTTCATTGGT
541      ACTTTTGAGG ACAAGACTTA CATTTGCAAA ACCACCATTG GGGACAGGGA AGTGGACTCC
601      GATACTTACT ATGTCTACAG CCTGCAGGTG TCATCCATCA ATGTCTCTGT GAATGCAGTG
661      CAGACTGTGG TCCGCCAGGG CGAGAGCATC ACCATCAGGT GCATCGTGAT GGGCAATGAT
721      GTGGTCAACT TCCAGTGGAC GTACCCCCGC ATGAAGAGTG GGCGGCTGGT GGAGCCGGTG
781      ACAGATTACC TCTTCGGAGT GCCCTCCCGC ATTGGCTCCA TTCTTCATAT CCCCACCGCT
841      GAGCTGAGTG ACTCTGGCAC CTATACTTGC AACGTGTCAG TGAGTGTGAA TGACCACGGC
901      GATGAGAAAG CCATCAACGT TACTGTGATC GAAAATGGCT ATGTGCGGCT GCTGGAAACA
961      CTGGAAGACG TACAAATTGC TGAGCTGCAC CGGAGCCGGA CGTTGCAGGT GGTGTTTGAG
1021     GCTTATCCGA CGCCCTCTGT CCTGTGGTTC AAGGACAACC GTACCTTGGG TGACTCCAGC
1081     GCTGGCGAGT TGGTTCTGTC CACTCGCAAC GTGTCTGAGA CCCGGTATGT GTCAGAACTG
1141     ACCTTGGTAC GTGTGAAGGT GTCAGAAGCG GGCTACTATA CCATGCGGGC CTTCCATGCG
1201     GACGACCAGG TCCAGCTCTC ATTCAAGCTG CAGGTCAATG TCCCTGTCCG TGTACTGGAG
1261     CTGAGTGAGA GTCATCCTGC CAATGGGGAG CAGATACTCC GCTGTCGGGG CCGGGGCATG
1321     CCTCAGCCAA ATGTCACCTG GTCTACCTGC AGAGACCTCA AAAGGTGTCC GCGAAAACTG
1381     TCACCCACAC CCTTGGGGAA TAGTTCCAAG GAGGAGAGCC AGCTAGAAAC GAACGTGACT
1441     TTCTGGGAGG AAGATCAGGA ATATGAGGTG GTGAGCACGC TGCGCCTGCG TCACGTGGAC
1501     CAGCCACTGT CAGTGCGCTG TATGCTGCAG AACTCGATGG GCAGAGATTC ACAAGAGGTC
1561     ACCGTGGTAC CACACTCCTT GCCTTTCAAA GTGGTGGTGA TCTCAGCCAT CTTGGCCTTG
1621     GTGGTCCTTA CTGTCATCTC TCTCATCATC CTCATCATGC TGTGGCAGAG GAAACCACGC
1681     TATGAGATCC GATGGAAGGT CATTGAGTCT GTGAGCTCTG ACGGCCATGA GTACATCTAT
1741     GTGGATCCTG TGCAGTTGCC TTACGACTCC ACCTGGGAGC TGCCACGGGA CCAGCTTGTT
1801     CTGGGACGCA CTCTTGGCTC TGGGGCTTTC GGACAGGTGG TGGAGGCCAC GGCTCATGGT
1861     CTGAGCCACT CACAGGCCAC CATGAAAGTG GCTGTCAAGA TGCTGAAATC TACAGCCAGA
1921     AGCAGTGAGA AGCAAGCCCT CATGTCGGAG CTGAAGATCA TGAGTCATCT CGGACCCCAC
1981     CTGAACGTGG TCAACCTGCT GGGGGCCTGT ACCAAAGGAG GGCCCATCTA CATTATCACG
2041     GAATACTGCC GTTATGGTGA CCTGGTGGAC TACCTGCACC GAAACAAACA CACCTTCCTG
2101     CAACGACACT CCAACAAGCA TTGTCCACCC AGCACTGAGC TCTACAGCAA CGCCCTGCCG
2161     GTGGGGCTCT CCCTACCCAG CCACTTGAAC CTGACTGGGG AGAGCGACGG TGGTTACATG
2221     GACATGAGCA AGGATGAATC TGTAGATTAC GTGCCCATGT TGGACATGAA AGGACACATC
2281     AAATACGCGG ACATCGAGTC CTCCAGCTAC ATGGCCCCTT ACGATAACTA TGTCCCATCT
2341     GCCCCTGAAA GGACCTATCG TGCTACCTTA ATCAACGACT CACCAGTGCT CAGCTACACG
2401     GACCTCGTGG GCTTCAGTTA CCAAGTGGCC AATGGCATGG AATTCTTGGC CTCTAAGAAC
2461     TGTGTTCACC GAGACCTGGC GGCCAGGAAT GTGCTCATCT GTGAGGGCAA GTTGGTCAAG
2521     ATCTGTGACT TTGGCCTGGC TCGAGACATC ATGCGGGACT CAAACTACAT CTCCAAAGGC
2581     AGTACTTTCC TGCCTCTGAA GTGGATGGCC CCAGAGAGCA TCTTCAACAG CCTCTACACC
2641     ACCTTGAGTG ATGTGTGGTC CTTTGGAATC CTACTCTGGG AAATCTTCAC ACTGGGTGGC
2701     ACCCCTTACC CAGAGCTGCC CATGAACGAC CAGTTCTACA ATGCCATCAA GAGGGGCTAC
2761     CGCATGGCCC AACCTGCTCA TGCCTCCGAC GAGATCTATG AGATCATGCA GAAATGCTGG
2821     GAAGAAAAGT TTGAGACTCG GCCCCCCTTC TCTCAGCTGG TGCTGCTCCT GGAGAGGCTT
2881     CTGGGTGAAG GCTATAAAAA GAAGTACCAG CAGGTGGATG AGGAGTTTCT GAGGAGTGAC
2941     CATCCAGCCA TCCTGAGGTC CCAAGCCCGC TTGCCGGGTC TCCACAGCCT CCGATCCCCT
3001     CTGGATACCA GCTCTGTCCT CTACACTGCT GTGCAGCCTA ATGAGACTGA CAATGACTAC
3061     ATCATCCCCT TGCCTGACCC CAAGCCTGAT GCTGCTGATG AGGGCCTCCT AGAGGGTTCC
3121     CCCAGCCTTG CCAGCTCCAC CTTGAATGAA GTCAACACTT CTTCCACCAT CTCCTGCGAC
3181     AGCCCCCTGG AGCTCCAAGA AGAGCCGCAA GCAGAGCCTG AGGCACAGCT GGAGCAGCCA
3241     CAGGATTCAG GCTGCCCAGG ACCTCTGGCT GAGGCAGAGG ACAGCTTCCT GTAG

 

Quality Control:cDNA sequencing results is available upon request.