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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat PDGFRB Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80347-G-F|
|Rat PDGFRB Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80347-G-H|
|Rat PDGFRB Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80347-G-M|
|Rat PDGFRB Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80347-G-N|
|Rat PDGFRB Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80347-G-Y|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.