PDGFRB / CD140b Antibody

PDGFRB / CD140b Antibody Datasheet

PDGFRB / CD140b Antibody Product Information

• Immunogen: Recombinant Human PDGFRB / CD140b protein (Catalog#10514-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 4D5D6
• Ig Type: Mouse IgG2a
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PDGFRB / CD140b (rh PDGFRB / CD140b; Catalog#10514-H08H; NP_002600.1; Met 1-Lys 531). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

PDGFRB / CD140b Antibody Usage Guide

• Specificity: Human PDGFRB / CD140b
• Immunochemistry: IHC-P: 10-30 μg/mL

  

  Immunochemical staining of human PDGFRb in human brain with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel endothelium.

  Immunochemical staining of human PDGFRb in human liver with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel endothelium.

  

  Immunochemical staining of human PDGFRb in human mammary adenocarcinoma with mouse monoclonal antibody (15 μg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to blood vessel endothelium.

• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PDGFRB / CD140b Antibody Background

Beta-type platelet-derived growth factor receptor, also known as CD140b, PDGFRB, PDGF-R-beta, PDGF receptor-β, is a member of theprotein kinase superfamily and CSF-1/PDGF receptor subfamily. The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four PDGFs are inactive in theirmonomericforms. The PDGFs bind to the proteintyrosine kinase receptors PDGF receptor-α and -β. These two receptor isoformsdimerizeupon binding the PDGF dimer, leading to three possible receptor combinations, namely -αα, -ββ and -αβ. Theextracellularregion of the PDGF receptor-βconsists of fiveimmunoglobulin-like domains while the intracellular part is atyrosine kinasedomain. The ligand-binding sites of PDGF receptor-α and -β are located to the three first immunoglobulin-like domains. PDGF-CC specifically interacts with PDGFR-αα and -αβ, but not with-ββ, and thereby resembles PDGF-AB. PDGF-DD binds to PDGFR-ββ with high affinity, and to PDGFR-αβ to a much lower extent and is regarded as PDGFR-ββ specific. PDGF-AA binds only to PDGFR-αα, while PDGF-BB is the only PDGF that can bind all three receptor combinations with high affinity.

References

1. Chi, KD. et al., 1997, Oncogene 15: 1051-7.
2. Arcaro, A. et al., 2000, Mol. Cell. Biol. 20: 3817-30.
3. Bergsten, E. et al., 2001, Nat. Cell Biol. 3: 512-16.
4. Zhang, Z. et al., 2004, Protein Sci. 13: 2819-24.
5. Morerio, C. et al., 2004, Cancer Res. 64: 2649-51.