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Human PDGFRα Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG10556-M |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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Manufactured By: Sino Biological Inc.
Gene Bank Ref.ID: NM_006206.4
cDNA Size: 3270
Synonym: CD140A; PDGFR2; MGC74795; Rhe-PDGFRA
Source: Human.
cDNA Description: ORF Clone of Homo sapiens platelet-derived growth factor receptor, alpha polypeptide DNA.
Vector:
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 516 C/T, 1701 A/G, 3222 T/C not causing the amino acid variation.
5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:
1 ATGGGGACTT CCCATCCGGC GTTCCTGGTC TTAGGCTGTC TTCTCACAGG GCTGAGCCTA 61 ATCCTCTGCC AGCTTTCATT ACCCTCTATC CTTCCAAATG AAAATGAAAA GGTTGTGCAG 121 CTGAATTCAT CCTTTTCTCT GAGATGCTTT GGGGAGAGTG AAGTGAGCTG GCAGTACCCC 181 ATGTCTGAAG AAGAGAGCTC CGATGTGGAA ATCAGAAATG AAGAAAACAA CAGCGGCCTT 241 TTTGTGACGG TCTTGGAAGT GAGCAGTGCC TCGGCGGCCC ACACAGGGTT GTACACTTGC 301 TATTACAACC ACACTCAGAC AGAAGAGAAT GAGCTTGAAG GCAGGCACAT TTACATCTAT 361 GTGCCAGACC CAGATGTAGC CTTTGTACCT CTAGGAATGA CGGATTATTT AGTCATCGTG 421 GAGGATGATG ATTCTGCCAT TATACCTTGT CGCACAACTG ATCCCGAGAC TCCTGTAACC 481 TTACACAACA GTGAGGGGGT GGTACCTGCC TCCTATGACA GCAGACAGGG CTTTAATGGG 541 ACCTTCACTG TAGGGCCCTA TATCTGTGAG GCCACCGTCA AAGGAAAGAA GTTCCAGACC 601 ATCCCATTTA ATGTTTATGC TTTAAAAGCA ACATCAGAGC TGGATCTAGA AATGGAAGCT 661 CTTAAAACCG TGTATAAGTC AGGGGAAACG ATTGTGGTCA CCTGTGCTGT TTTTAACAAT 721 GAGGTGGTTG ACCTTCAATG GACTTACCCT GGAGAAGTGA AAGGCAAAGG CATCACAATG 781 CTGGAAGAAA TCAAAGTCCC ATCCATCAAA TTGGTGTACA CTTTGACGGT CCCCGAGGCC 841 ACGGTGAAAG ACAGTGGAGA TTACGAATGT GCTGCCCGCC AGGCTACCAG GGAGGTCAAA 901 GAAATGAAGA AAGTCACTAT TTCTGTCCAT GAGAAAGGTT TCATTGAAAT CAAACCCACC 961 TTCAGCCAGT TGGAAGCTGT CAACCTGCAT GAAGTCAAAC ATTTTGTTGT AGAGGTGCGG 1021 GCCTACCCAC CTCCCAGGAT ATCCTGGCTG AAAAACAATC TGACTCTGAT TGAAAATCTC 1081 ACTGAGATCA CCACTGATGT GGAAAAGATT CAGGAAATAA GGTATCGAAG CAAATTAAAG 1141 CTGATCCGTG CTAAGGAAGA AGACAGTGGC CATTATACTA TTGTAGCTCA AAATGAAGAT 1201 GCTGTGAAGA GCTATACTTT TGAACTGTTA ACTCAAGTTC CTTCATCCAT TCTGGACTTG 1261 GTCGATGATC ACCATGGCTC AACTGGGGGA CAGACGGTGA GGTGCACAGC TGAAGGCACG 1321 CCGCTTCCTG ATATTGAGTG GATGATATGC AAAGATATTA AGAAATGTAA TAATGAAACT 1381 TCCTGGACTA TTTTGGCCAA CAATGTCTCA AACATCATCA CGGAGATCCA CTCCCGAGAC 1441 AGGAGTACCG TGGAGGGCCG TGTGACTTTC GCCAAAGTGG AGGAGACCAT CGCCGTGCGA 1501 TGCCTGGCTA AGAATCTCCT TGGAGCTGAG AACCGAGAGC TGAAGCTGGT GGCTCCCACC 1561 CTGCGTTCTG AACTCACGGT GGCTGCTGCA GTCCTGGTGC TGTTGGTGAT TGTGATCATC 1621 TCACTTATTG TCCTGGTTGT CATTTGGAAA CAGAAACCGA GGTATGAAAT TCGCTGGAGG 1681 GTCATTGAAT CAATCAGCCC GGATGGACAT GAATATATTT ATGTGGACCC GATGCAGCTG 1741 CCTTATGACT CAAGATGGGA GTTTCCAAGA GATGGACTAG TGCTTGGTCG GGTCTTGGGG 1801 TCTGGAGCGT TTGGGAAGGT GGTTGAAGGA ACAGCCTATG GATTAAGCCG GTCCCAACCT 1861 GTCATGAAAG TTGCAGTGAA GATGCTAAAA CCCACGGCCA GATCCAGTGA AAAACAAGCT 1921 CTCATGTCTG AACTGAAGAT AATGACTCAC CTGGGGCCAC ATTTGAACAT TGTAAACTTG 1981 CTGGGAGCCT GCACCAAGTC AGGCCCCATT TACATCATCA CAGAGTATTG CTTCTATGGA 2041 GATTTGGTCA ACTATTTGCA TAAGAATAGG GATAGCTTCC TGAGCCACCA CCCAGAGAAG 2101 CCAAAGAAAG AGCTGGATAT CTTTGGATTG AACCCTGCTG ATGAAAGCAC ACGGAGCTAT 2161 GTTATTTTAT CTTTTGAAAA CAATGGTGAC TACATGGACA TGAAGCAGGC TGATACTACA 2221 CAGTATGTCC CCATGCTAGA AAGGAAAGAG GTTTCTAAAT ATTCCGACAT CCAGAGATCA 2281 CTCTATGATC GTCCAGCCTC ATATAAGAAG AAATCTATGT TAGACTCAGA AGTCAAAAAC 2341 CTCCTTTCAG ATGATAACTC AGAAGGCCTT ACTTTATTGG ATTTGTTGAG CTTCACCTAT 2401 CAAGTTGCCC GAGGAATGGA GTTTTTGGCT TCAAAAAATT GTGTCCACCG TGATCTGGCT 2461 GCTCGCAACG TCCTCCTGGC ACAAGGAAAA ATTGTGAAGA TCTGTGACTT TGGCCTGGCC 2521 AGAGACATCA TGCATGATTC GAACTATGTG TCGAAAGGCA GTACCTTTCT GCCCGTGAAG 2581 TGGATGGCTC CTGAGAGCAT CTTTGACAAC CTCTACACCA CACTGAGTGA TGTCTGGTCT 2641 TATGGCATTC TGCTCTGGGA GATCTTTTCC CTTGGTGGCA CCCCTTACCC CGGCATGATG 2701 GTGGATTCTA CTTTCTACAA TAAGATCAAG AGTGGGTACC GGATGGCCAA GCCTGACCAC 2761 GCTACCAGTG AAGTCTACGA GATCATGGTG AAATGCTGGA ACAGTGAGCC GGAGAAGAGA 2821 CCCTCCTTTT ACCACCTGAG TGAGATTGTG GAGAATCTGC TGCCTGGACA ATATAAAAAG 2881 AGTTATGAAA AAATTCACCT GGACTTCCTG AAGAGTGACC ATCCTGCTGT GGCACGCATG 2941 CGTGTGGACT CAGACAATGC ATACATTGGT GTCACCTACA AAAACGAGGA AGACAAGCTG 3001 AAGGACTGGG AGGGTGGTCT GGATGAGCAG AGACTGAGCG CTGACAGTGG CTACATCATT 3061 CCTCTGCCTG ACATTGACCC TGTCCCTGAG GAGGAGGACC TGGGCAAGAG GAACAGACAC 3121 AGCTCGCAGA CCTCTGAAGA GAGTGCCATT GAGACGGGTT CCAGCAGTTC CACCTTCATC 3181 AAGAGAGAGG ACGAGACCAT TGAAGACATC GACATGATGG ACGACATCGG CATAGACTCT 3241 TCAGACCTGG TGGAAGACAG CTTCCTGTAA
Quality Control: cDNA sequencing results is available upon request.
PDGFRA/CD140a related areas, pathways, and other information
