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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human PDGFD ORF mammalian expression plasmid, C-GFPSpark tag||HG13232-ACG|
|Human PDGFD ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13232-ACR|
|Human PDGFD ORF mammalian expression plasmid, C-Flag tag||HG13232-CF|
|Human PDGFD ORF mammalian expression plasmid, C-His tag||HG13232-CH|
|Human PDGFD ORF mammalian expression plasmid, C-Myc tag||HG13232-CM|
|Human PDGFD ORF mammalian expression plasmid, C-HA tag||HG13232-CY|
|Human PDGFD ORF mammalian expression plasmid, N-Flag tag||HG13232-NF|
|Human PDGFD ORF mammalian expression plasmid, N-His tag||HG13232-NH|
|Human PDGFD ORF mammalian expression plasmid, N-Myc tag||HG13232-NM|
|Human PDGFD ORF mammalian expression plasmid, N-HA tag||HG13232-NY|
|Human PDGFD natural ORF mammalian expression plasmid||HG13232-UT|
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Platelet-derived growth factor D (PDGF-D), also known as Iris-expressed growth factor, is a member of the PDGF/vascular endothelial growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. PDGF-D/PDGFD only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. The expression of PDGF-D/PDGFD in the eye is tissue-specific. In the anterior segment, it is localized to iris and ciliary body, whereas in the retina, PDGF-D/PDGFD is restricted to the outer plexiform layer. PDGF-D/PDGFD is present in aqueous humor but is not detectable in mature lens or in mouse lens-derived alphaTN4-1 cells. PDGF-D/PDGFD is highly expressed in human breast cancer and facilitates tumor growth and lymph node metastasis, making it a potential target in breast cancer. PDGF-D/PDGFD increases drug delivery and hence improves the efficacy of chemotherapy through vessel normalization. Intervention in the PDGF-D pathway in the eye, perhaps by antibody or blocking peptide, could be useful in the treatment of certain cataracts, including post-operative secondary cataract.