Mouse PDGFD Gene cDNA Clone / ORF Clone

PDGFD cDNA Clone Datasheet

PDGFD cDNA Clone Product Information

• Gene Bank Ref.ID: NM_027924.2
• cDNA Size: 1113
• Gene Synonym: 1110003I09Rik; Pdgfd
• Source: Mouse

PDGFD cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus platelet-derived growth factor, D polypeptide DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PDGFD cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDGFD cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDGFD cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAACGGC TCGTTTTAGT CTCCATTCTC CTGTGCGCGA ACTTTAGCTG CTATCCGGAC
61       ACTTTTGCGA CTCCGCAGAG AGCATCCATC AAAGCTTTGC GCAATGCCAA CCTCAGGAGA
121      GATGAGAGCA ATCACCTCAC AGACTTGTAC CAGAGAGAGG AGAACATTCA GGTGACAAGC
181      AATGGCCATG TGCAGAGTCC TCGCTTCCCG AACAGCTACC CAAGGAACCT GCTTCTGACA
241      TGGTGGCTCC GTTCCCAGGA GAAAACACGG ATACAACTGT CCTTTGACCA TCAATTCGGA
301      CTAGAGGAAG CAGAAAATGA CATTTGTAGG TATGACTTTG TGGAAGTTGA AGAAGTCTCA
361      GAGAGCAGCA CTGTTGTCAG AGGAAGATGG TGTGGCCACA AGGAGATCCC TCCAAGGATA
421      ACGTCAAGAA CAAACCAGAT TAAAATCACA TTTAAGTCTG ATGACTACTT TGTGGCAAAA
481      CCTGGATTCA AGATTTATTA TTCATTTGTG GAAGATTTCC AACCGGAAGC AGCCTCAGAG
541      ACCAACTGGG AATCAGTCAC AAGCTCTTTC TCTGGGGTGT CCTATCACTC TCCATCAATA
601      ACGGACCCCA CTCTCACTGC TGATGCCCTG GACAAAACTG TCGCAGAATT CGATACCGTG
661      GAAGATCTAC TTAAGCACTT CAATCCAGTG TCTTGGCAAG ATGATCTGGA GAATTTGTAT
721      CTGGACACCC CTCATTATAG AGGCAGGTCA TACCATGATC GGAAGTCCAA AGTGGACCTG
781      GACAGGCTCA ATGATGATGT CAAGCGTTAC AGTTGCACTC CCAGGAATCA CTCTGTGAAC
841      CTCAGGGAGG AGCTGAAGCT GACCAATGCA GTCTTCTTCC CACGATGCCT CCTCGTGCAG
901      CGCTGTGGTG GCAACTGTGG TTGCGGAACT GTCAACTGGA AGTCCTGCAC ATGCAGCTCA
961      GGGAAGACAG TGAAGAAGTA TCATGAGGTA TTGAAGTTTG AGCCTGGACA TTTCAAGAGA
1021     AGGGGCAAAG CTAAGAATAT GGCTCTTGTT GATATCCAGC TGGATCATCA TGAGCGATGT
1081     GACTGTATCT GCAGCTCAAG ACCACCTCGA TAA

 

Quality Control:cDNA sequencing results is available upon request.