Human PDE6B Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13626-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PDE6B cDNA Clone Datasheet

PDE6B cDNA Clone Product Information

Gene Bank Ref.ID:	BC000249
cDNA Size:	2562
Gene Synonym:	rd1;PDEB;RP40;CSNB3;CSNBAD2;PDE6B
Source:	Human

PDE6B cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens phosphodiesterase 6B, cGMP-specific, rod, beta DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PDE6B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDE6B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDE6B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCCTCA GTGAGGAGCA GGCCCGGAGC TTTCTGGACC AGAACCCCGA TTTTGCCCGC
61       CAGTACTTTG GGAAGAAACT GAGCCCTGAG AATGTGGCCG CGGCCTGCGA GGACGGGTGC
121      CCGCCGGACT GCGACAGCCT CCGGGACCTC TGCCAGGTGG AGGAGAGCAC GGCGCTGCTG
181      GAGCTGGTGC AGGATATGCA GGAGAGCATC AACATGGAGC GCGTGGTCTT CAAGGTCCTG
241      CGGCGCCTCT GCACCCTCCT GCAGGCCGAC CGCTGCAGCC TCTTCATGTA CCGCCAGCGC
301      AACGGCGTGG CCGAGCTGGC CACCAGGCTT TTCAGCGTGC AGCCGGACAG CGTCCTGGAG
361      GACTGCCTGG TGCCCCCCGA CTCCGAGATC GTCTTCCCAC TGGACATCGG GGTCGTGGGC
421      CACGTGGCTC AGACCAAAAA GATGGTGAAC GTCGAGGACG TGGCCGAGTG CCCTCACTTC
481      AGCTCATTTG CTGACGAGCT CACTGACTAC AAGACAAAGA ATATGCTGGC CACACCCATC
541      ATGAATGGCA AAGACGTCGT GGCGGTGATC ATGGCAGTGA ACAAGCTCAA CGGCCCATTC
601      TTCACCAGCG AAGACGAAGA TGTGTTCTTG AAGTACCTGA ATTTTGCCAC GTTGTACCTG
661      AAGATCTATC ACCTGAGCTA CCTCCACAAC TGCGAGACGC GCCGCGGCCA GGTGCTGCTG
721      TGGTCGGCCA ACAAGGTGTT TGAGGAGCTG ACGGACATCG AGAGGCAGTT CCACAAGGCC
781      TTCTACACGG TGCGGGCCTA CCTCAACTGC GAGCGGTACT CCGTGGGCCT CCTGGACATG
841      ACCAAGGAGA AGGAATTTTT TGACGTGTGG TCTGTGCTGA TGGGAGAGTC CCAGCCGTAC
901      TCGGGCCCAC GCACGCCTGA TGGCCGGGAA ATTGTCTTCT ACAAAGTGAT CGACTACATC
961      CTCCACGGCA AGGAGGAGAT CAAGGTCATT CCCACACCCT CAGCCGATCA CTGGGCCCTG
1021     GCCAGCGGCC TTCCAAGCTA CGTGGCAGAA AGCGGCTTTA TTTGTAACAT CATGAATGCT
1081     TCCGCTGACG AAATGTTCAA ATTTCAGGAA GGGGCCCTGG ACGACTCCGG GTGGCTCATC
1141     AAGAATGTGC TGTCCATGCC CATCGTCAAC AAGAAGGAGG AGATTGTGGG AGTCGCCACA
1201     TTTTACAACA GGAAAGACGG GAAGCCCTTT GACGAACAGG ACGAGGTTCT CATGGAGTCC
1261     CTGACACAGT TCCTGGGCTG GTCAGTGATG AACACCGACA CCTACGACAA GATGAACAAG
1321     CTGGAGAACC GCAAGGACAT CGCACAGGAC ATGGTCCTTT ACCACGTGAA GTGCGACAGG
1381     GACGAGATCC AGCTCATCCT GCCAACCAGA GCGCGCCTGG GGAAGGAGCC TGCTGACTGC
1441     GATGAGGACG AGCTGGGCGA AATCCTGAAG GAGGAGCTGC CAGGGCCCAC CACATTTGAC
1501     ATCTACGAAT TCCACTTCTC TGACCTGGAG TGCACCGAAC TGGACCTGGT CAAATGTGGC
1561     ATCCAGATGT ACTACGAGCT GGGCGTGGTC CGAAAGTTCC AGATCCCCCA GGAGGTCCTG
1621     GTGCGGTTCC TGTTCTCCAT CAGCAAAGGG TACCGGAGAA TCACCTACCA CAACTGGCGC
1681     CACGGCTTCA ACGTGGCCCA GACGATGTTC ACGCTGCTCA TGACCGGCAA ACTGAAGAGC
1741     TACTACACGG ACCTGGAGGC CTTCGCCATG GTGACAGCCG GCCTGTGCCA TGACATCGAC
1801     CACCGCGGCA CCAACAACCT GTACCAGATG AAGTCCCAGA ACCCCTTGGC TAAGCTCCAC
1861     GGCTCCTCGA TTTTGGAGCG GCACCACCTG GAGTTTGGGA AGTTCCTGCT CTCGGAGGAG
1921     ACCCTGAACA TCTACCAGAA CCTGAACCGG CGGCAGCACG ATCACGTGAT CCACCTGATG
1981     GACATCGCCA TCATCGCCAC GGACCTGGCC CTGTACTTCA AGAAGAGAGC GATGTTTCAG
2041     AAGATCGTGG ATGAGTCCAA GAACTACCAG GACAAGAAGA GCTGGGTGGA GTACCTGTCC
2101     CTGGAGACGA CCCGGAAGGA GATCGTCATG GCCATGATGA TGACAGCCTG CGACCTGTCT
2161     GCCATCACCA AGCCCTGGGA AGTCCAGAGC AAGGTCGCAC TTCTCGTGGC TGCTGAGTTC
2221     TGGGAGCAAG GTGACTTGGA AAGGACAGTC TTGGATCAGC AGCCCATTCC TATGATGGAC
2281     CGGAACAAGG CGGCCGAGCT CCCCAAGCTG CAAGTGGGCT TCATCGACTT CGTGTGCACA
2341     TTCGTGTACA AGGAGTTCTC TCGTTTCCAC GAAGAGATCC TGCCCATGTT CGACCGACTG
2401     CAGAACAATA GGAAAGAGTG GAAGGCGCTG GCTGATGAGT ATGAGGCCAA AGTGAAGGCT
2461     CTGGAGGAGA AGGAGGAGGA GGAGAGGGTG GCAGCCAAGA AAGGCACAGA AATTTGCAAT
2521     GGCGGCCCAG CACCCAAGTC TTCAACCTGC TGTATCCTGT GA

Quality Control:cDNA sequencing results is available upon request.