Human PDE4D Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12138-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PDE4D cDNA Clone Datasheet

PDE4D cDNA Clone Product Information

Gene Bank Ref.ID:	BC008390
cDNA Size:	660
Gene Synonym:	DPDE3; PDE43; STRK1; HSPDE4D; PDE4DN2; FLJ97311; DKFZp686M11213;PDE4D
Source:	Human

PDE4D cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens phosphodiesterase 4D, cAMP-specific (phosphodieste) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PDE4D cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PDE4D cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PDE4D cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTCAGC AGACAAGCCC GGACACTTTA ACAGTACCTG AAGTGGATAA TCCGCATTGT
61       CCAAACCCGT GGCTGAACGA AGACCTTGTG AAATCCTTGC GAGAAAACCT GTTGCAGCAT
121      GAGAAGTCCA AGACAGCGAG GAAATCGGTT TCTCCCAAGC TCTCTCCAGT GATCTCTCCG
181      AGAAATTCCC CCAGGCTTCT GCGCAGAATG CTTCTCAGCA GCAACATCCC CAAACAGCGG
241      CGTTTCACGG TGGCACATAC ATGTTTTGAT GTGGACAATG GCACATCTGC GGGACGGAGT
301      CCCTTGGATC CCATGACCAG CCCAGGATCC GGGCTAATTC TCCAAGCAAA TTTTGTCCAC
361      AGTCAACGAC GGGAGTCCTT CCTGTATCGA TCCGACAGCG ATTATGACCT CTCTCCAAAG
421      TCTATGTCCC GGAACTCCTC CATTGCCAGT GATATACACG GAGATGACTT GATTGTGACT
481      CCATTTGCTC AGGTCTTGGC CAGTCTGCGA ACTGTACGAA ACAACTTTGC TGCATTAACT
541      AATTTGCAAG ATCGAGCACC TAGCAAAAGA TCACCCATGT GCAACCAACC ATCCATCAAC
601      AAAGCCACCA TAACAGGATC CTGGATGGAA TTGAACCCAT ATACGTTACT TGACATGTGA

Quality Control:cDNA sequencing results is available upon request.