Human PD1 / PDCD1 ORF Expression Clone, FLAG-tagged

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10377-M-F	pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PD1 / PDCD1 cDNA Clone Datasheet

PD1 / PDCD1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_005018.2
cDNA Size:	894
Gene Synonym:	PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l
Source:	Human

PD1 / PDCD1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens programmed cell death 1 (PDCD1) DNA.
Vector:	pCMV/hygro-FLAG
FLAG Tag Sequence:	GATTACAAGGATGACGACGATAAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

PD1 / PDCD1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PD1 / PDCD1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PD1 / PDCD1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAGATCC CACAGGCGCC CTGGCCAGTC GTCTGGGCGG TGCTACAACT GGGCTGGCGG
61       CCAGGATGGT TCTTAGACTC CCCAGACAGG CCCTGGAACC CCCCCACCTT CTCCCCAGCC
121      CTGCTCGTGG TGACCGAAGG GGACAACGCC ACCTTCACCT GCAGCTTCTC CAACACATCG
181      GAGAGCTTCG TGCTAAACTG GTACCGCATG AGCCCCAGCA ACCAGACGGA CAAGCTGGCC
241      GCCTTCCCCG AGGACCGCAG CCAGCCCGGC CAGGACTGCC GCTTCCGTGT CACACAACTG
301      CCCAACGGGC GTGACTTCCA CATGAGCGTG GTCAGGGCCC GGCGCAATGA CAGCGGCACC
361      TACCTCTGTG GGGCCATCTC CCTGGCCCCC AAGGCGCAGA TCAAAGAGAG CCTGCGGGCA
421      GAGCTCAGGG TGACAGAGAG AAGGGCAGAA GTGCCCACAG CCCACCCCAG CCCCTCACCC
481      AGGCCAGCCG GCCAGTTCCA AACCCTGGTG GTTGGTGTCG TGGGCGGCCT GCTGGGCAGC
541      CTGGTGCTGC TAGTCTGGGT CCTGGCCGTC ATCTGCTCCC GGGCCGCACG AGGGACAATA
601      GGAGCCAGGC GCACCGGCCA GCCCCTGAAG GAGGACCCCT CAGCCGTGCC TGTGTTCTCT
661      GTGGACTATG GGGAGCTGGA TTTCCAGTGG CGAGAGAAGA CCCCGGAGCC CCCCGTGCCC
721      TGTGTCCCTG AGCAGACGGA GTATGCCACC ATTGTCTTTC CTAGCGGAAT GGGCACCTCA
781      TCCCCCGCCC GCAGGGGCTC AGCTGACGGC CCTCGGAGTG CCCAGCCACT GAGGCCTGAG
841      GATGGACACT GCTCTTGGCC CCTCGCT¹GAT TACAAGGATG ACGACGATAA G²TAA

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

Quality Control:cDNA sequencing results is available upon request.