PD-L1 Protein & Antibody (B7-H1, CD274)

Programmed Death-Ligand 1 (Cluster of Differentiation 274)

PD-L1 Products

PD-L1 Protein, Recombinant

Molecule	Species	Description	Cat. No
PD-L1/B7-H1/CD274	Human	PD-L1/B7-H1/CD274/Fc Protein, Recombinant	10084-H02H
PD-L1/B7-H1/CD274	Human	PD-L1/B7-H1/CD274 Protein, Recombinant	10084-H08H
PD-L1/B7-H1/CD274	Mouse	PD-L1/B7-H1/CD274/Fc Protein, Recombinant	50010-M03H
PD-L1/B7-H1/CD274	Mouse	PD-L1/B7-H1/CD274 Protein, Recombinant	50010-M08H

  10084-H02H: Immobilized human PD1 at 10µg/ml (100µl/well) can bind recombinant human B7-H1/PD-L1/Fc chimera with a linear range of 0.02-0.4 µg/ml. Measured by its binding ability in a functional ELISA.

  10084-H08H: Immobilized human B7-H1 at 20 µg/ml (100 µl/well) can bind human PD1 with a linear ranger of 0.032-0.8 µg/ml. Measured by its binding ability in a functional ELISA.

  50010-M03H:  Immobilized recombinant mouse PD-1 at 10 µg/ml (100 µl/well) can bind biotinylated mouse PD-L1 with a linear range of 3.2-400 ng/ml. Measured by its binding ability in a functional Elisa.

  50010-M08H:  Immobilized recombinant mouse PD-1 at 1 µg/ml (100 µl/well) can bind biotinylated mouse PD-L1 with a linear range of 6.25-400 ng/ml. Measured by its binding ability in a functional Elisa.

PD-L1 Antibody

Molecule	Application	Description	Cat. No
Human PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Monoclonal Antibody	10084-R001
Human PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Polyclonal Antibody	10084-RP01
Mouse PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Polyclonal Antibody	50010-RP01
Mouse PD-L1/B7-H1/CD274	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	50010-RP02

PD-L1 Alternative Names

PD-L1, B7H1, CD274, B7-H, PDCD1L1, PDCD1LG1, PDL1, MGC142294, MGC142296 [Homo sapiens]

PD-L1, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1, Cd274 A530045L16Rik [Mus musculus]

PD-L1 Related Areas

Immunology>>Adaptive Immunity>>Costimulation & Costimulatory Molecule>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Other>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Other>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>Natural Killer (NK) Cell CD Antigen>>PD-L1/B7-H1/CD274

Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Other>>PD-L1/B7-H1/CD274

PD-L1 Background

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

PD-L1 Related Studies

1. Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
2. Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
3. Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
4. Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
5. Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.