PD-L1 / B7-H1 / CD274 Antibody

PD-L1 / B7-H1 / CD274 Antibody Datasheet

PD-L1 / B7-H1 / CD274 Antibody Product Information

• Immunogen: Recombinant Mouse PD-L1 / B7-H1 / CD274 protein (Catalog#50010-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 401
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PD-L1 / B7-H1 / CD274 (rM PD-L1 / B7-H1 / CD274; Catalog#50010-M08H; NP_068693.1; Met 1-Thr 238).

PD-L1 / B7-H1 / CD274 Antibody Usage Guide

• Specificity: Mouse PD-L1 / B7-H1 / CD274
• Flow Cytometry:
  

  Flow cytometric analysis of CD274 expression on spleen lymphocytes. BALB/c splenocytes were stained with Mouse CD274 Monoclonal Ab (50010-R401, 1 μg/test), then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of lymphocytes.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PD-L1 / B7-H1 / CD274 Antibody Background

B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between B7-H1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. As a costimulator, B7-H1 induces T-cell proliferation and preferentially the production of interleukin 10 and interferon gamma. Conversely, by binding to programmed death 1 receptors (PD-1) on activated T-cells and B-cells, B7-H1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, B7-H1 leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. B7-H1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

References

1. Dong, H. et al., 1999, Nat. Med. 5: 1365-1369.
2. Freeman, G.J. et al., 2000, J. Exp. Med. 192:1027-103.
3. Dong, H., et al., 2002, Nat. Med. 8: 793-800.
4. Tamura, H. et al., 2003, Int. J. Hematol. 78: 321-328.
5. Trabattoni, D. et al., 2003, Blood. 101: 2514-2520.