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PD-L1 / B7-H1 / CD274 Antibody, Rabbit MAb

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Human CD274 Antibody Product Information
Immunogen:Recombinant Human PD-L1 / B7-H1 / CD274 protein (Catalog#10084-H08H)
Clone ID:015
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human PD-L1 / B7-H1 / CD274 (rh PD-L1 / B7-H1 / CD274; Catalog#10084-H08H; NP_054862.1; Met 1-Thr 239).
Human CD274 Antibody Usage Guide
Specificity:Human PD-L1 / B7-H1 / CD274
No cross-reactivity in ELISA with
Human B7-1
Human B7-2
Mouse B7-H1
Non-blocking
This B7-H1/PD-L1 antibody can not block the binding of recombinant PD1 (10377-H03H) to its recombinant ligand B7-H1/PD-L1 (10084-H02H).
Application:ELISA, IHC-P

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human B7H1.

IHC-P: 5-15 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD274 Antibody IHC Application Image
PD-L1 / B7-H1 / CD274 Antibody, Rabbit MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human B7H1 in human placenta with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to trophoblast.
Other CD274 Antibody Products
Reactivity: Human  
Application: ELISA  IHC-P  
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10084-T24-200
10084-T24-100
10084-T24-50
200 µg 
100 µg 
50 µg 
Add to Cart
Reactivity: Human  
Application: B/N  
    10084-R639-500
    10084-R639-200
    500 µg 
    200 µg 
    Add to Cart
    Reactivity: Human  
    Application: IHC-P  
    • Slide 1
    • Slide 1
    • Slide 1
    10084-MB55-100
    10084-MB55-50
    10084-MB55-200
    100 µg 
    50 µg 
    200 µg 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      10084-MM02-100
      10084-MM02-50
      10084-MM02-200
      100 µg 
      50 µg 
      200 µg 
      Add to Cart
      Reactivity: Human  
      Application: ELISA  
        10084-RP01-100
        10084-RP01-200
        100 µg 
        200 µg 
        Add to Cart
        Reactivity: Human  
        Application: ELISA  
          10084-R001-50
          10084-R001-100
          50 µg 
          100 µg 
          Add to Cart
          Reactivity: Human  
          Application: ELISA  
            10084-RP02-50
            10084-RP02-100
            50 µg 
            100 µg 
            Add to Cart
            Reactivity: Human  
            Application: ELISA  
              10084-MM13-100
              10084-MM13-50
              100 µg 
              50 µg 
              Add to Cart
              Reactivity: Human  
              Application: ELISA  
                10084-T16-50
                10084-T16-100
                50 µg 
                100 µg 
                Add to Cart
                Reactivity: Mouse  
                Application: FCM  IF  ICC/IF  
                • Slide 1
                • Slide 1
                50010-R485-100
                50010-R485-50
                100 µg 
                50 µg 
                Add to Cart
                Reactivity: Mouse  
                Application: ELISA  
                  50010-RP01-400
                  50010-RP01-100
                  50010-RP01-200
                   
                  100 µg 
                  200 µg 
                  Add to Cart
                  Reactivity: Mouse  
                  Application: ELISA  
                    50010-RP02-200
                    50010-RP02-50
                    50010-RP02-100
                    200 µg 
                    50 µg 
                    100 µg 
                    Add to Cart
                    Reactivity: Mouse  
                    Application: FCM  
                    • Slide 1
                    50010-R401-50
                    50010-R401-100
                    50 µg 
                    100 µg 
                    Add to Cart
                    Reactivity: Mouse  
                    Application: FCM  
                    • Slide 1
                    50010-R485-P-50
                    50010-R485-P-100
                    50 Tests 
                    100 Tests 
                    Add to Cart
                    Reactivity: Mouse  
                    Application: FCM  
                    • Slide 1
                    50010-R485-A-50
                    50010-R485-A-100
                    50 Tests 
                    100 Tests 
                    Add to Cart
                    Reactivity: Cynomolgus  
                    Application: ELISA  WB  
                    • Slide 1
                    90251-T48-200
                    90251-T48-100
                    90251-T48-50
                    200 µg 
                    100 µg 
                    50 µg 
                    Add to Cart
                    PD-L1/B7-H1/CD274 Background

                    Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

                    Human PD-L1/B7-H1/CD274 References
                  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
                  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
                  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
                  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
                  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.
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                    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"