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PD-L1 / B7-H1 / CD274 Antibody, Rabbit MAb

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Human B7-H1/PD-L1 Antibody Product Information
Immunogen:Recombinant Human B7-H1 protein (Catalog#10084-H08H)
Clone ID:1
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human B7-H1 (rh B7-H1; Catalog#10084-H08H; NP_054862.1; Met 1-Thr 239) .
Human B7-H1/PD-L1 Antibody Usage Guide
Specificity:Human B7-H1 / CD274 / PD-L1
No cross-reactivity in ELISA with
Mouse B7-H1 / CD274 / PD-L1
Human B7-1 / CD80 / CD28LG1
Human B7-2 / CD86 / CD28LG2
Non-blocking
This B7-H1/PD-L1 antibody can not block the binding of recombinant PD1 (10377-H03H) to its recombinant ligand B7-H1/PD-L1 (10084-H02H).
Application:ELISA

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human B7-H1. The detection limit for Human B7-H1 is approximately 0.00975 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other B7-H1/PD-L1 Antibody Products
Reactivity : Human  
Application : ELISA  IHC-P  
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10084-T24-200
10084-T24-100
10084-T24-50
200 µg 
100 µg 
50 µg 
Add to Cart
Reactivity : Human  
Application : B/N  
    10084-R639-500
    10084-R639-200
    500 µg 
    200 µg 
    Add to Cart
    Reactivity : Human  
    Application : ELISA  IHC-P  
    • Slide 1
    10084-R015-50
    10084-R015-100
    50 µg 
    100 µg 
    Add to Cart
    Reactivity : Human  
    Application : IHC-P  
    • Slide 1
    • Slide 1
    • Slide 1
    10084-MB55-100
    10084-MB55-50
    10084-MB55-200
    100 µg 
    50 µg 
    200 µg 
    Add to Cart
    Reactivity : Human  
    Application : ELISA  
      10084-MM02-100
      10084-MM02-50
      10084-MM02-200
      100 µg 
      50 µg 
      200 µg 
      Add to Cart
      Reactivity : Human  
      Application : ELISA  
        10084-RP01-100
        10084-RP01-200
        100 µg 
        200 µg 
        Add to Cart
        Reactivity : Human  
        Application : ELISA  
          10084-RP02-50
          10084-RP02-100
          50 µg 
          100 µg 
          Add to Cart
          Reactivity : Human  
          Application : ELISA  
            10084-MM13-100
            10084-MM13-50
            100 µg 
            50 µg 
            Add to Cart
            Reactivity : Human  
            Application : ELISA  
              10084-T16-50
              10084-T16-100
              50 µg 
              100 µg 
              Add to Cart
              Reactivity : Mouse  
              Application : FCM  IF  ICC/IF  
              • Slide 1
              • Slide 1
              50010-R485-100
              50010-R485-50
              100 µg 
              50 µg 
              Add to Cart
              Reactivity : Mouse  
              Application : ELISA  
                50010-RP01-400
                50010-RP01-100
                50010-RP01-200
                 
                100 µg 
                200 µg 
                Add to Cart
                Reactivity : Mouse  
                Application : ELISA  
                  50010-RP02-200
                  50010-RP02-50
                  50010-RP02-100
                  200 µg 
                  50 µg 
                  100 µg 
                  PD-L1/B7-H1/CD274 Background

                  Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

                  Human PD-L1/B7-H1/CD274 References
                • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
                • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
                • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
                • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
                • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.
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                  Size / Price
                  Catalog: 10084-R001-50
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                  All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                  Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"