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Human PD-ECGF Gene cDNA Clone (full-length ORF Clone)
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Human PD-ECGF Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG10432-M





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

Antibody Production Services

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:  NM_001953.3

cDNA Size:   1449

Synonym:  TP; ECGF1; MNGIE; PDECGF; hPD-ECGF; TYMP

Source:  Human.

cDNA Description:  ORF Clone of Homo sapiens thymidine phosphorylase DNA.

Vector: Cloning Vector: pMD18-T Simple Vector.

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG


Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for the point mutation 972 C/T not causing the amino acid variation.

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .
Sequence:

1        ATGGCAGCCT TGATGACCCC GGGAACCGGG GCCCCACCCG CGCCTGGTGA CTTCTCCGGG
61       GAAGGGAGCC AGGGACTTCC CGACCCTTCG CCAGAGCCCA AGCAGCTCCC GGAGCTGATC
121      CGCATGAAGC GAGACGGAGG CCGCCTGAGC GAAGCGGACA TCAGGGGCTT CGTGGCCGCT
181      GTGGTGAATG GGAGCGCGCA GGGCGCACAG ATCGGGGCCA TGCTGATGGC CATCCGACTT
241      CGGGGCATGG ATCTGGAGGA GACCTCGGTG CTGACCCAGG CCCTGGCTCA GTCGGGACAG
301      CAGCTGGAGT GGCCAGAGGC CTGGCGCCAG CAGCTTGTGG ACAAGCATTC CACAGGGGGT
361      GTGGGTGACA AGGTCAGCCT GGTCCTCGCA CCTGCCCTGG CGGCATGTGG CTGCAAGGTG
421      CCAATGATCA GCGGACGTGG TCTGGGGCAC ACAGGAGGCA CCTTGGATAA GCTGGAGTCT
481      ATTCCTGGAT TCAATGTCAT CCAGAGCCCA GAGCAGATGC AAGTGCTGCT GGACCAGGCG
541      GGCTGCTGTA TCGTGGGTCA GAGTGAGCAG CTGGTTCCTG CGGACGGAAT CCTATATGCA
601      GCCAGAGATG TGACAGCCAC CGTGGACAGC CTGCCACTCA TCACAGCCTC CATTCTCAGT
661      AAGAAACTCG TGGAGGGGCT GTCCGCTCTG GTGGTGGACG TTAAGTTCGG AGGGGCCGCC
721      GTCTTCCCCA ACCAGGAGCA GGCCCGGGAG CTGGCAAAGA CGCTGGTTGG CGTGGGAGCC
781      AGCCTAGGGC TTCGGGTCGC GGCAGCGCTG ACCGCCATGG ACAAGCCCCT GGGTCGCTGC
841      GTGGGCCACG CCCTGGAGGT GGAGGAGGCG CTGCTCTGCA TGGACGGCGC AGGCCCGCCA
901      GACTTAAGGG ACCTGGTCAC CACGCTCGGG GGCGCCCTGC TCTGGCTCAG CGGACACGCG
961      GGGACTCAGG CTCAGGGCGC TGCCCGGGTG GCCGCGGCGC TGGACGACGG CTCGGCCCTT
1021     GGCCGCTTCG AGCGGATGCT GGCGGCGCAG GGCGTGGATC CCGGTCTGGC CCGAGCCCTG
1081     TGCTCGGGAA GTCCCGCAGA ACGCCGGCAG CTGCTGCCTC GCGCCCGGGA GCAGGAGGAG
1141     CTGCTGGCGC CCGCAGATGG CACCGTGGAG CTGGTCCGGG CGCTGCCGCT GGCGCTGGTG
1201     CTGCACGAGC TCGGGGCCGG GCGCAGCCGC GCTGGGGAGC CGCTCCGCCT GGGGGTGGGC
1261     GCAGAGCTGC TGGTCGACGT GGGTCAGAGG CTGCGCCGTG GGACCCCCTG GCTCCGCGTG
1321     CACCGGGACG GCCCCGCGCT CAGCGGCCCG CAGAGCCGCG CCCTGCAGGA GGCGCTCGTA
1381     CTCTCCGACC GCGCGCCATT CGCCGCCCCC TCGCCCTTCG CAGAGCTCGT TCTGCCGCCG
1441     CAGCAATAA


Quality Control:   cDNA sequencing results is available upon request.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"