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Human PD-ECGF Gene cDNA Clone (full-length ORF Clone)

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PD-ECGF/TYMPcDNA Clone Product Information
Gene Bank Ref.ID:NM_001953.3
cDNA Size:1449
cDNA Description:ORF Clone of Homo sapiens thymidine phosphorylase DNA.
Gene Synonym:TP, ECGF1, MNGIE, PDECGF, hPD-ECGF, TYMP
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutation 972 C/T not causing the amino acid variation.
Shipping Carrier:Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.
Storage:The Whatman FTA elute card can be stored at room temperature for three months under dry condition.
pMD18-T  Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
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Catalog:HG10432-M
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