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Product Catalog
PCSK9 ELISA Pair Set, Rat ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK80005 |
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Rat PCSK9 ELISA Pair Set
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Order or Inquire for PCSK9 ELISA Pair Set |
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High affinity Rat PCSK9 ELISA Pair Set |
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Detection limit - 47 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Rat PCSK9 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Rat proprotein convertase subtilisin / kexin type 9 ( PCSK9 ), also known as NARC1 ( neural apoptosis regulated convertase ), is a newly identified human secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the generation of an active proteinase with a broad alkaline pH optimum and no apparent calcium requirement for activity. PCSK9 is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. PCSK9 is able to bind directly to the LDLR through its epidermal growth factor-like repeat A (EGF-A) binding domain, and induces and accelerates LDLR internalization and degradation. Accordingly, PCSK9 contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with autosomal dominant hypercholesterolemia characterized by increased LDL cholesterol level, which is a risk factor for coronary heart disease.
