PCSK9 Matched ELISA Antibody Pair Set,Rat
Solid Phase Sandwich ELISA
Quantitative determination of Rat PCSK9
1. Capture Antibody 0.5 mg/mL of mouse anti-Rat PCSK9/NARC1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 80005-MM06)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Rat PCSK9/NARC1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 80005-R007)
3. Standard Each vial contains 23 ng of recombinant Rat PCSK9/NARC1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1500 pg/mL is recommended.
This PCSK9 Matched ELISA Antibody Pair Set,Rat is a solid phase sandwich ELISA for quantitative determination of Rat PCSK9 . It contains Rat PCSK9 capture antibody, Rat PCSK9 detector antibody
and a highly purified
recombinant Rat PCSK9 protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.
Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498 Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.