PCSK9 Antibody

PCSK9 Antibody Datasheet

PCSK9 Antibody Product Information

• Immunogen: Recombinant Mouse PCSK9 protein (Catalog#50251-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 106
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PCSK9 (rM PCSK9; Catalog#50251-M08H; NP_705793.1; Met 1-Gln 694).

PCSK9 Antibody Usage Guide

• Specificity: Mouse PCSK9
• No cross-reactivity in ELISA with

  Human PCSK9

  Rhesus PCSK9

  Human PCSK1

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse PCSK9. The detection limit for Mouse PCSK9 is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

PCSK9 Antibody Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), is a newly identified human secretory subtilase belonging to the proteinase K subfamily. PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the generation of an active proteinase with a broad alkaline pH optimum and no apparent calcium requirement for activity. PCSK9 is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. PCSK9 is able to bind directly to the LDLR through its epidermal growth factor-like repeat A (EGF-A) binding domain, and induces and accelerates LDLR internalization and degradation. Accordingly, PCSK9 contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with autosomal dominant hypercholesterolemia characterized by increased LDL cholesterol level, which is a risk factor for coronary heart disease.

References

1. Sseidah, N.G. et al., 2003, Proc. Natl. Acad. Sci. USA. 100: 928-933.
2. Beyer, T.P. et al., 2007, J. Lipid. Res. 48: 1488-1498.
3. Shan, L. et al., 2008, Biochem. Biophys. Res. Commun. 375: 69-73.
4. Benjannet, S. et al., 2005, J. Biol. Chem. 279: 48865-48875.
5. Abifadel, M. et al., 2003, Nat. Genet. 34: 154-156.