Mouse PCSK1 Gene cDNA Clone / ORF Clone

PCSK1 cDNA Clone Datasheet

PCSK1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013628.2
• cDNA Size: 2262
• Gene Synonym: PC1; PC3; Nec1; SPC3; Nec-1; Phpp-1; MGC124320; MGC124321; Pcsk1
• Source: Mouse

PCSK1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus proprotein convertase subtilisin/kexin type 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence except for the point mutation 237 C/T not causing the amino acid variation.

PCSK1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PCSK1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PCSK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGCAAA GAGGTTGGAC TCTGCAGTGT ACTGCTTTCG CCTTCTTTTG CGTTTGGTGT
61       GCACTAAACA GTGTAAAAGC AAAGAGGCAG TTTGTTAATG AATGGGCGGC GGAGATCCCC
121      GGAGGGCAAG AAGCTGCCTC TGCCATCGCC GAAGAACTGG GGTATGACCT TTTGGGTCAG
181      ATTGGATCAC TTGAAAATCA CTATTTATTC AAACACAAAA GCCATCCTCG GAGGTCTCGA
241      AGAAGCGCTC TTCATATCAC TAAGAGGTTA TCTGATGATG ATCGTGTGAC GTGGGCTGAA
301      CAACAGTATG AAAAAGAGAG AAGTAAACGT TCAGTTCAAA AAGACTCAGC ATTGGATCTC
361      TTCAATGATC CAATGTGGAA TCAGCAGTGG TACTTGCAAG ATACCAGAAT GACTGCAGCT
421      CTGCCCAAGC TGGACCTTCA TGTAATACCT GTTTGGGAAA AGGGTATTAC TGGCAAAGGA
481      GTTGTTATTA CTGTACTGGA TGATGGCTTG GAGTGGAATC ACACAGACAT TTATGCCAAT
541      TATGATCCAG AGGCTAGCTA TGATTTTAAC GATAATGATC ATGATCCATT TCCCCGATAT
601      GATCTCACAA ATGAAAACAA ACATGGAACA AGATGTGCAG GTGAAATTGC CATGCAAGCA
661      AATAATCACA AGTGTGGGGT TGGAGTTGCA TATAATTCCA AAGTTGGAGG CATAAGAATG
721      CTGGATGGCA TTGTAACTGA TGCCATTGAG GCTAGTTCAA TTGGATTCAA CCCTGGCCAT
781      GTGGATATTT ACAGTGCAAG CTGGGGCCCT AATGATGATG GAAAAACTGT GGAGGGGCCT
841      GGCAGACTAG CCCAGAAGGC ATTTGAATAT GGTGTCAAAC AGGGGAGACA AGGGAAAGGC
901      TCCATCTTTG TCTGGGCTTC AGGGAATGGG GGTCGTCAGG GAGATAACTG TGACTGTGAT
961      GGCTACACAG ACAGCATTTA CACCATCTCT ATCAGCAGTG CCTCCCAGCA AGGCCTGTCA
1021     CCTTGGTATG CAGAGAAGTG TTCTTCCACA TTGGCTACCT CCTACAGCAG TGGTGATTAC
1081     ACAGACCAGC GAATAACAAG CGCTGACCTG CACAATGACT GCACAGAGAC CCACACAGGC
1141     ACCTCGGCTT CAGCACCCCT GGCTGCTGGT ATCTTTGCTC TGGCCTTGGA GGCAAACCCA
1201     AATCTTACCT GGAGAGATAT GCAGCATCTG GTTGTCTGGA CCTCTGAGTA CGACCCATTG
1261     GCCAGTAACC CAGGTTGGAA AAAGAATGGG GCAGGCTTGA TGGTGAACAG CCGATTTGGA
1321     TTTGGCTTGC TAAATGCCAA AGCTCTGGTG GATTTGGCTG ATCCTAGGAC CTGGAGAAAT
1381     GTGCCTGAGA AGAAAGAATG TGTTGTAAAA GACAATAACT TTGAGCCTAG AGCCCTGAAA
1441     GCTAATGGAG AAGTAATTGT TGAAATCCCA ACAAGAGCTT GTGAAGGACA AGAAAATGCT
1501     ATCAAGTCTC TGGAACATGT GCAATTTGAA GCAACAATTG AATATTCTCG TAGAGGAGAC
1561     CTTCATGTCA CACTCACTTC TGCTGTTGGA ACCAGCACTG TACTGTTGGC TGAAAGGGAA
1621     AGAGATACAT CCCCCAATGG CTTTAAGAAT TGGGACTTCA TGTCTGTTCA TACATGGGGA
1681     GAGAATCCTG TAGGCACCTG GACATTGAAA ATTACAGACA TGTCTGGAAG AATGCAAAAT
1741     GAAGGAAGGA TTGTGAACTG GAAGTTGATT TTGCATGGGA CATCTTCTCA ACCAGAGCAC
1801     ATGAAGCAGC CTCGTGTGTA CACATCCTAC AATACAGTCC AGAATGACAG GAGAGGAGTG
1861     GAAAAGATGG TGAATGTTGT GGAGAAGCGG CCCACACAAA AGAGCCTGAA TGGCAATCTC
1921     CTGGTACCCA AAAACTCCAG CAGCAGCAAT GTGGAGGGTA GAAGGGATGA GCAGGTACAA
1981     GGAACTCCTT CAAAGGCCAT GCTGCGACTC CTACAAAGTG CTTTTAGCAA GAATGCACTT
2041     TCAAAACAAT CACCAAAGAA GTCTCCAAGT GCAAAGCTCA GCATCCCTTA TGAAAGTTTC
2101     TATGAAGCCT TGGAAAAGCT CAACAAGCCC TCCAAGCTTG AAGGCTCTGA AGACAGTCTG
2161     TACAGTGACT ATGTTGATGT ATTCTATAAC ACAAAACCTT ATAAGCATAG AGATGACAGG
2221     CTGCTGCAAG CTCTCATGGA CATCCTAAAT GAGGAGAATT AA

 

Quality Control:cDNA sequencing results is available upon request.