Human PCOLCE2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13476-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PCOLCE2 cDNA Clone Datasheet

PCOLCE2 cDNA Clone Product Information

Gene Bank Ref.ID:	BC006265
cDNA Size:	831
Gene Synonym:	PCPE2; PCOLCE2
Source:	Human

PCOLCE2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens procollagen C-endopeptidase enhancer 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PCOLCE2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PCOLCE2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PCOLCE2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGGGCG CGAACGCCTG GGCGCCACTC TGCCTGCTGC TGGCTGCCGC CACCCAGCTC
61       TCGCGGCAGC AGTCCCCAGA GAGACCTGTT TTCACATGTG GTGGCATTCT TACTGGAGAG
121      TCTGGATTTA TTGGCAGTGA AGGTTTTCCT GGAGTGTACC CTCCAAATAG CAAATGTACT
181      TGGAAAATCA CAGTTCCCGA AGGAAAAGTA GTCGTTCTCA ATTTCCGATT CATAGACCTC
241      GAGAGTGACA ACCTGTGCCG CTATGACTTT GTGGATGTGT ACAATGGCCA TGCCAATGGC
301      CAGCGCATTG GCCGCTTCTG TGGCACTTTC CGGCCTGGAG CCCTTGTGTC CAGTGGCAAC
361      AAGATGATGG TGCAGATGAT TTCTGATGCC AACACAGCTG GCAATGGCTT CATGGCCATG
421      TTCTCCGCTG CTGAACCAAA CGAAAGAGGG GATCAGTATT GTGGAGGACT CCTTGACAGA
481      CCTTCCGGCT CTTTTAAAAC CCCCAACTGG CCAGACCGGG ATTACCCTGC AGGAGTCACT
541      TGTGTGTGGC ACATTGTAGC CCCAAAGAAT CAGCTTATAG AATTAAAGTT TGAGAAGTTT
601      GATGTGGAGC GAGATAACTA CTGCCGATAT GATTATGTGG CTGTGTTTAA TGGCGGGGAA
661      GTCAACGATG CTAGAAGAAT TGGAAAGTAT TGTGGTGATA GTCCACCTGC GCCAATTGTG
721      TCTGAGAGAA ATGAACTTCT TATTCAGTTT TTATCAGACT TAAGTTTAAC TGCAGATGGG
781      TTTATTGGTC ACTACATATT CAGGCCAAAA AAACTGCCTA CAACTACATA A

Quality Control:cDNA sequencing results is available upon request.