PCNA Protein Price Inquiry ( Available Sizes )
PCNA Protein Product Information
||A DNA sequence encoding the mature form of human PCNA (P12004) (Met 1-Ser 261) was expressed, with a polyhistidine tag at the N-terminus.
PCNA Protein QC Testing
||> 90 % as determined by SDS-PAGE.
||< 1.0 EU per μg of the protein as determined by the LAL method
||Samples are stable for up to twelve months from date of receipt at -70℃
|Predicted N terminal:
||The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions.
||Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10%glycerol, pH 7.0, 2mM DTT
- Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
- Please contact us for any concerns or special requirements.
PCNA Protein Usage Guide
||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
PCNA Protein Related Products & Topics
PCNA Protein Description
The DNA polymerase processivity factor proliferating cell nuclear antigen (PCNA) is central to both DNA replication and repair. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. Proliferating cell nuclear antigen (PCNA) is a processivity factor for DNA polymerases δ and ɛ (pol δ and ɛ) and is an essential protein for replication of chromosomal DNA. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA pol δ and ε onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery (4) indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.
- Balajee AS. et al., 2001, Nucleic acids Res. 29 (6): 1341-51.
- Ducoux M. et al., 2001, J Biol Chem. 276 (52): 49258-66.
- Tetsuo I. et al., 2002, P Genes Cells. 7 (10): 997-1007.
PCNA related areas, pathways, and other information