PCNA Protein (His Tag)

Proliferating cell nuclear antigen Protein Datasheet

PCNA Protein Product Information

• Synonym: MGC8367
• Protein Construction: A DNA sequence encoding the mature form of human PCNA (P12004) (Met 1-Ser 261) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

PCNA Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PCNA consists of 280 amino acids and has a calculated molecular mass of 31KDa. It migrates as an approximately 36 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Na3PO4, 300mM NaCl, 10%glycerol, pH 7.0, 2mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PCNA protein

PCNA Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PCNA Protein Description

The DNA polymerase processivity factor proliferating cell nuclear antigen (PCNA) is central to both DNA replication and repair. Two forms of PCNA exist in cells: (i) a detergent-insoluble trimeric form stably associated with the replicating forks during S phase and (ii) a soluble form in quiescent cells in G1 and G2 phases. Proliferating cell nuclear antigen (PCNA) is a processivity factor for DNA polymerases δ and ɛ (pol δ and ɛ) and is an essential protein for replication of chromosomal DNA. PCNA forms a toroidal trimer in S phase with replication factor-C (RF-C) and DNA in an ATP-dependent manner and enables the loading of DNA pol δ and ε onto the complex. The close association of PCNA with kinase complexes involved in cell cycle machinery (4) indicates that PCNA has a regulatory role in cell cycle progression. PCNA also participates in the processing of branched intermediates that arise during the lagging strand DNA synthesis.

References

1. Balajee AS. et al., 2001, Nucleic acids Res. 29 (6): 1341-51.
2. Ducoux M. et al., 2001, J Biol Chem. 276 (52): 49258-66.
3. Tetsuo I. et al., 2002, P Genes Cells. 7 (10): 997-1007.