Human PCMT1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13841-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PCMT1 cDNA Clone Datasheet

PCMT1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC007501
cDNA Size:	687
Gene Synonym:	PIMT,PCMT1
Source:	Human

PCMT1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protein-L-isoaspartate (D-aspartate) O-methyltransferase DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

PCMT1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PCMT1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PCMT1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCTGGA AATCCGGCGG CGCCAGCCAC TCGGAGCTAA TCCACAATCT CCGCAAAAAT
61       GGAATCATCA AGACAGATAA AGTATTTGAA GTGATGCTGG CTACAGACCG CTCCCACTAT
121      GCAAAATGTA ACCCATACAT GGATTCTCCA CAATCAATAG GTTTCCAAGC AACAATCAGT
181      GCTCCACACA TGCATGCATA TGCGCTAGAA CTTCTATTTG ATCAGTTGCA TGAAGGAGCT
241      AAAGCTCTTG ATGTAGGATC TGGAAGTGGA ATCCTTACTG CATGTTTTGC ACGTATGGTT
301      GGATGTACTG GAAAAGTCAT AGGAATTGAT CACATTAAAG AGCTAGTAGA TGACTCAGTA
361      AATAATGTCA GGAAGGACGA TCCAACACTT CTGTCTTCAG GGAGAGTACA GCTTGTTGTG
421      GGGGATGGAA GAATGGGATA TGCTGAAGAA GCCCCTTATG ATGCCATTCA TGTGGGAGCT
481      GCAGCCCCTG TTGTACCCCA GGCGCTAATA GATCAGTTAA AGCCCGGAGG AAGATTGATA
541      TTGCCTGTTG GTCCTGCAGG CGGAAACCAA ATGTTGGAGC AGTATGACAA GCTACAAGAT
601      GGCAGCATCA AAATGAAGCC TCTGATGGGG GTGATATACG TGCCTTTAAC AGATAAAGAA
661      AAGCAGTGGT CCAGGGATGA ATTGTAA

Quality Control:cDNA sequencing results is available upon request.