Human PCDHGB3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13396-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PCDHGB3 cDNA Clone Datasheet

PCDHGB3 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_032097.1
cDNA Size:	2445
Gene Synonym:	PCDH-GAMMA-B3; PCDHGB3
Source:	Human

PCDHGB3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protocadherin gamma subfamily B, 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 83 T/C resulting in the amino acid Val substitution by Ala.

PCDHGB3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PCDHGB3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PCDHGB3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAAATA GCTCCGGATG GAGGGGCCCA GCAGGGCAGA GGCGAATGCT ATTTCTCTTC
61       CTGCTCTCTT TGTTAGACCA GGCTCTCTCC GAACCGATCC GCTACGCTAT TCCCGAGGAG
121      CTGGACAGGG GCTCGCTGGT AGGGAACCTC GCCAAGGACC TGGGGTTTGG CGTGGGGGAT
181      TTACCTACTA GGAACCTGCG GGTTATTGCA GAGAAGAAAT TCTTTACCGT GAGCCCCGAA
241      AATGGGAACT TACTTGTGAG CGACCGTATA GACCGAGAGG AGATTTGTGG CAAGAAGTCG
301      ACGTGTGTTC TGGAATTTGA AATGGTTGCT GAAAAGCCTT TAAACTTTTT TCATGTAACT
361      GTGCTGATCC AGGATATTAA CGACAACCCA CCGACCTTTA GCCAAAATAT CACTGAGCTG
421      GAAATCAGCG AACTGGCTCT CACTGGAGCC ACATTTGCCC TGGAATCTGC GCAAGATCCT
481      GATGTAGGTG TCAATTCGCT GCAGCAGTAC TACCTCAGCC CTGATCCGCA CTTCTCTTTG
541      ATTCAGAAGG AGAACCTGGA TGGCAGTAGG TACCCAGAGC TAGTACTGAA AGCACCCCTG
601      GACAGGGAAG AGCAGCCACA TCACCACCTG GTCCTCACAG CTGTGGATGG GGGCGAGCCC
661      TCCAGAAGCT GTACCACCCA GATCAGGGTA ATTGTCGCAG ATGCAAATGA TAACCCCCCA
721      GTATTTACTC AGGACATGTA CAGGGTCAAT GTTGCAGAGA ACCTGCCCGC TGGCTCCTCC
781      GTATTAAAAG TGATGGCCAT TGACATGGAT GAGGGCATCA ATGCCGAAAT CATCTATGCC
841      TTCATCAATA TTGGCAAGGA AGTGAGACAA CTGTTCAAGC TGGACAGTAA AACGGGGGAA
901      CTCACCACTA TTGGAGAACT GGACTTTGAA GAGAGAGATA GCTACACAAT TGGGGTGGAA
961      GCAAAGGATG GTGGACATCA CACTGCATAT TGTAAAGTAC AGATAGATAT TTCAGATGAA
1021     AATGACAATG CCCCGGAGAT AACCCTGGCT TCTGAATCCC AACATATACA AGAAGATGCT
1081     GAGCTGGGGA CTGCCGTTGC CCTGATCAAA ACACATGATC TAGATTCTGG ATTTAATGGA
1141     GAAATCCTAT GCCAACTAAA AGGAAACTTC CCCTTTAAAA TCGTTCAAGA TACCAAAAAC
1201     ACATACAGGT TGGTGACAGA TGGAGCCCTG GACCGGGAGC AGATCCCAGA ATACAATGTG
1261     ACGATCACAG CTACCGACAA AGGCAATCCA CCGCTCTCCT CCAGCAAGAC CATCACTCTG
1321     CACATCCTTG ATGTCAACGA CAACGTTCCC GTTTTCCACC AGGCCTCCTA CACCGTGCAT
1381     GTAGCTGAGA ACAATCCGCC TGGAGCCTCC ATTGCGCATG TCAGAGCCTC GGATCCCGAC
1441     TTGGGACCTA ATGGCCTTGT CTCCTACTAC ATCGTGGCCA GTGACCTGGA GCCGCGGGAG
1501     CTGTCGTCCT ACGTGTCCGT GAGCGCGCGG AGCGGGGTGG TGTTCGCGCA GCGAGCCTTC
1561     GACCACGAGC AGCTGCGTGC CTTCGAGCTC ACTCTGCAGG CCCGCGACCA GGGCTCGCCT
1621     ACGCTCAGCG CCAACGTGAG CCTGCGCGTG TTGGTGGACG ACCGCAACGA CAATGCACCG
1681     CTGGTGCTGT ACCCAGCTCT GGGGCCCGAA GGCTCTGCGC TCTTCGATAT GGTGCCGCGC
1741     TCTGCAGAGC CTGGCTACCT GGTGACCAAG GTGGTGGCGG TGGACGCAGA CTCGGGATAC
1801     AACGCCTGGC TGTCCTACCA CATTGTGCAG GCCAGCGAGC CCGGGCTGTT CAGCCTGGGC
1861     CTGCGCACGG GTGAGGTGCG CACGGCGCGT ACCTTGGGCG ACAGGGAGGC CGCCCGCCAG
1921     CGCCTGCTGG TCACTGTGCG TGATGGAGGA CAGCAGCCTC TTTCAGCCAC CGTCATGCTG
1981     CACCTAATCT TCGCAGATAG CTTGCAAGAG ATACAACCTG ACCTTAGCGA CCGCCCCACT
2041     CCCTCTGACC CTCAGGCGGA GCTACAGTTT CACCTAGTAG TGGCGTTGGC CTTGATCTCA
2101     GTGCTCTTCC TCCTCGCGGT GATTCTGGCA ATCTCCCTGC GCCTGCGATG CTCCTCCAGA
2161     CCCGCCACTG AGGGCTACTT TCAGCCTGGT GTCTGCTTCA AGACTGTACC TGGAGTTCTC
2221     CCCACCTACA GCGAAAGGAC TTTGCCTTAT TCCTACAATC CGTGTGCTGC CTCACATTCC
2281     TCAAACACCG AGTTTAAATT TCTCAATATA AAGGCTGAAA ATGCTGCACC ACAAGATCTT
2341     CTATGTGATG AAGCCTCTTG GTTTGAAAGT AATGACAATC CAGAAATGCC TTCTAATTCA
2401     GGCAATTTGC AAAAGGTGAG TTTCTTCAAA CCTTTCCTTC CATAA

Quality Control:cDNA sequencing results is available upon request.