Human PCDHGA5 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13390-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PCDHGA5 cDNA Clone Datasheet

PCDHGA5 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_032054.1
cDNA Size:	2442
Gene Synonym:	ME3; CDH-GAMMA-A5; PCDH-GAMMA-A5; PCDHGA5
Source:	Human

PCDHGA5 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protocadherin gamma subfamily A, 5 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 832 A/G resulting in the amino acid Thr substitution by Ala.

PCDHGA5 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PCDHGA5 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PCDHGA5 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGAGTC CACCTAGGGG CTGGGGCTGC GGAGAGCTGC TGCTGCCCTT CATGCTCCTG
61       GGGACGCTGT GCGAGCCAGG ATCCGGGCAG ATCCGCTACT CGATGCCGGA GGAGCTGGAC
121      AAAGGCTCCT TCGTCGGCAA CATAGCCAAG GACCTTGGGC TGGAGCCCCA GGAGCTGGCG
181      GAGCGCGGAG TCCGCATCGT CTCCAGAGGT AGGACGCAGC TTTTTGCCCT GAACCCGCGA
241      AGCGGCAGCT TGGTCACCGC GGGCAGGATA GACCGGGAGG AGCTCTGCGC TCAGAGCCCA
301      CTGTGTGTGG TGAACTTTAA CATCTTGGTT GAGAACAAAA TGAAAATTTA TGGAGTAGAA
361      GTAGAAATAA TCGATATTAA TGATAACTTC CCGCGTTTCC GGGATGAAGA GTTAAAAGTA
421      AAAGTTAATG AAAATGCGGC TGCAGGGACA CGGTTAGTGC TTCCCTTCGC GCGGGATGCG
481      GATGTGGGTG TGAACTCTCT CCGGAGTTAC CAGCTCAGCT CCAATCTGCA CTTCTCTCTG
541      GATGTGGTAA GCGGAACTGA TGGACAAAAG TATCCGGAGC TGGTGTTGGA ACAGCCCCTA
601      GACCGCGAGA AAGAGACTGT TCACGACCTC CTCCTCACAG CTTTAGATGG CGGAGACCCG
661      GTACTCTCCG GCACCACGCA CATCCGTGTT ACGGTCCTCG ACGCAAACGA CAATGCGCCC
721      CTGTTCACCC CATCCGAGTA CAGCGTGAGT GTTCCAGAGA ACATACCTGT GGGCACTCGG
781      CTGCTCATGC TAACCGCCAC GGATCCAGAT GAGGGAATAA ACGGGAAATT GGCCTACTCT
841      TTTCGCAATG AAGAAGAAAA AATTTCGGAG ACTTTCCAAC TTGATTCCAA CCTGGGGGAA
901      ATCTCAACTC TACAATCACT GGACTATGAA GAATCCAGAT TCTACCTCAT GGAAGTGGTA
961      GCTCAGGATG GAGGCGCTCT TGTTGCCAGC GCTAAGGTGG TGGTCACAGT ACAGGACGTG
1021     AATGACAATG CCCCCGAAGT GATCCTCACC TCTCTGACCA GTTCGATCTC TGAAGACTGT
1081     CTTCCCGGAA CTGTAATCGC GCTGTTTAGC GTACATGATG GTGATTCTGG AGAAAATGGT
1141     GAGATTGCAT GCTCTATTCC TAGGAATTTG CCTTTTAAAT TGGAGAAGTC AGTTGATAAT
1201     TACTATCACC TATTAACAAC TAGGGACCTG GACAGAGAAG AGACTTCAGA TTATAATATC
1261     ACTTTAACCG TCATGGACCA TGGAACCCCG CCCCTCTCTA CAGAAAGCCA CATCCCCTTG
1321     AAAGTAGCAG ACGTTAATGA CAACCCACCC AATTTCCCTC AAGCCTCCTA CTCCACCTCT
1381     GTCACAGAAA ACAATCCCAG AGGTGTCTCT ATCTTCTCTG TGACAGCCCA TGACCCCGAC
1441     AGCGGCGACA ACGCTCGAGT CACCTACTCC CTGGCTGAAG ACACATTTCA GGGGGCGCCC
1501     TTGTCCTCCT ATGTATCCAT TAACTCTGAC ACCGGTGTCC TGTATGCTCT GAGATCCTTC
1561     GACTATGAGC AGTTGAGAGA CCTACAGTTG TGGGTGACAG CCAGCGACAG TGGGAACCCT
1621     CCACTTAGCA GCAACGTGTC GCTGAGCCTG TTTGTGCTGG ACCAGAACGA CAATACGCCT
1681     GAGATCCTGT ACCCCGCCCT CCCCACAGAC GGTTCCACGG GCGTGGAGCT GGCGCCTCGC
1741     TCCGCAGAAC CTGGCTACCT GGTGACCAAG GTGGTAGCGG TGGACAAAGA TTCAGGCCAG
1801     AACGCCTGGC TGTCCTACCG CCTGCTTAAG GCCAGCGAGC CAGGACTCTT TGCGGTTGGG
1861     CTGCACACGG GCGAGGTGCG CACAGCGCGA GCCCTGCTGG ACAGAGACGC GCTCAAGCAG
1921     AGCCTCGTGG TGGCCGTCGA AGACCATGGC CAGCCCCCTC TGTCAGCCAC CTTCACGGTC
1981     ACCGTTGCCG TGGCCGACAG GATCCCTGAC ATCCTGGCTG ACCTAGGCAG TATCAAGACC
2041     CCCATTGACC CTGAGGATCT GGACCTCACA CTCTATCTTG TGGTGGCAGT GGCTGCAGTC
2101     TCCTGCGTCT TCCTGGCCTT CGTCATCGTG CTGCTGGTGC TCAGACTGAG GCGCTGGCAC
2161     AAGTCACGCC TGCTTCAGGC TGAAGGCAGC AGGTTGGCGG GTGTGCCCGC CTCGCACTTT
2221     GTGGGCGTGG ATGGGGTTCG GGCTTTCCTG CAGACCTATT CCCACGAGGT CTCCCTCACC
2281     GCGGACTCGA GGAAGAGTCA CCTGATCTTT CCCCAGCCCA ACTACGCAGA CACGCTCCTT
2341     AGTGAAGAGA GCTGTGAGAA AAGCGAGCCT CTTCTGATGT CTGATAAGGT AGATGCAAAC
2401     AAAGAAGAAC GGCGAGTTCA GGTTAGTTTT CTCTTTCGGT AA

Quality Control:cDNA sequencing results is available upon request.