Human PCDHGA1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13398-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PCDHGA1 cDNA Clone Datasheet

PCDHGA1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_031993.1
cDNA Size:	2472
Gene Synonym:	PCDH-GAMMA-A1; PCDHGA1
Source:	Human

PCDHGA1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protocadherin gamma subfamily A, 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 454 G/A resulting in the amino acid Val substitution by Ile.

PCDHGA1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PCDHGA1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PCDHGA1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGATTC AGAAAAAGCT GACTGGCTGC AGCAGGCTGA TGCTTCTGTG TCTTTCTCTG
61       GAGCTGCTGT TGGAAGCTGG GGCTGGGAAT ATTCACTACT CAGTGCCGGA AGAGACAGAC
121      AAAGGTTCCT TCGTAGGCAA CATCGCCAAG GACCTAGGGC TGCAACCCCA GGAGCTGGCA
181      GATGGCGGAG TCCGCATCGT CTCCAGAGGT AGGATGCCGC TTTTCGCTCT GAATCCTAGA
241      AGTGGCAGCT TGATCACCGC GCGCAGGATA GACCGGGAGG AGCTCTGCGC TCAGAGCATG
301      CCGTGTCTCG TGAGTTTTAA TATCCTTGTT GAGGATAAAA TGAAGCTTTT TCCTGTTGAA
361      GTAGAAATAA TTGATATTAA TGACAACACT CCCCAATTCC AGTTAGAGGA ACTGGAGTTT
421      AAAATGAATG AAATAACGAC TCCAGGTACC AGAATCTCAT TGCCTTTTGG GCAAGACCTT
481      GATGTGGGTA TGAACTCACT CCAGAGCTAC CAACTCAGCT CTAACCCTCA TTTCTCCCTG
541      GATGTGCAAC AGGGAGCCGA TGGGCCTCAA CATCCAGAGA TGGTGCTGCA GAGTCCCTTA
601      GACAGAGAAG AAGAAGCTGT CCACCACCTC ATCCTCACAG CTTCTGATGG GGGTGAACCA
661      GTCCGTTCAG GGACCCTCAG AATTTACATT CAGGTGGTGG ATGCAAATGA CAATCCTCCA
721      GCATTTACTC AGGCACAATA CCATATAAAT GTCCCCGAAA ACGTGCCGCT GGGTACTCAG
781      CTGCTCATGG TAAATGCCAC TGACCCTGAT GAGGGAGCCA ATGGGGAAGT AACGTACTCC
841      TTTCACAATG TAGACCACAG AGTGGCCCAA ATATTTCGTT TAGATTCTTA CACAGGAGAA
901      ATATCAAATA AAGAACCACT AGATTTCGAA GAATACAAAA TGTATTCAAT GGAAGTTCAA
961      GCCCAGGATG GTGCGGGGCT CATGGCTAAA GTTAAGGTAC TGATCAAAGT TTTGGATGTA
1021     AATGATAATG CCCCAGAAGT GACCATCACC TCTGTCACCA CTGCAGTTCC AGAAAACTTT
1081     CCTCCTGGGA CCATAATTGC TCTTATCAGT GTGCATGACC AGGACTCAGG AGACAATGGC
1141     TACACCACAT GTTTCATTCC TGGAAATTTA CCCTTTAAAT TGGAAAAGTT AGTTGATAAT
1201     TATTACCGTT TAGTGACTGA AAGAACACTG GACAGAGAAC TTATCTCTGG GTACAACATC
1261     ACAATAACAG CAATAGACCA AGGAACTCCA GCTCTATCTA CTGAAACTCA CATTTCACTA
1321     CTAGTGACAG ATATCAATGA CAACTCCCCA GTCTTCCATC AGGACTCCTA CTCTGCCTAC
1381     ATTCCCGAAA ACAACCCCAG AGGAGCCTCC ATCTTCTCTG TGAGGGCCCA CGACTTGGAC
1441     AGCAATGAGA ATGCACAAAT CACTTACTCC CTAATAGAGG ACACTATCCA GGGGGCACCC
1501     CTATCTGCCT ACCTCTCCAT CAACTCCGAC ACTGGGGTCC TGTATGCGCT GCGATCCTTC
1561     GACTATGAGC AGTTCCGGGA CATGCAACTG AAAGTGATGG CGCGGGACAG TGGGGATCCG
1621     CCCCTCAGCA GCAACGTGTC TCTCAGCCTA TTCCTGCTGG ACCAGAACGA CAACGCGCCC
1681     GAGATCCTGT ACCCCGCCCT CCCCACAGAT GGTTCTACCG GCGTGGAGCT GGCGCCCCTC
1741     TCCGCAGAGC CCGGCTACCT GGTGACCAAG GTGGTGGCGG TGGACAGAGA CTCGGGCCAG
1801     AACGCCTGGC TGTCCTACCG CCTGCTCAAG GCCAGCGAGC CGGGACTCTT CTCGGTGGGT
1861     CTGCACACGG GCGAGGTGCG CACGGCGCGA GCCCTGCTGG ACAGAGACGC GCTCAAGCAG
1921     AGTCTCGTGG TGGCCGTCCA GGACCACGGC CAGCCCCCGC TCTCCGCCAC TGTCACGCTC
1981     ACCGTGGCCG TGGCCGACAG GATCTCCGAC ATCCTGGCCG ACCTGGGCAG CCTCGAGCCC
2041     TCCGCCAAAC CCAACGATTC GGACCTCACT CTGTACCTGG TGGTGGCGGC GGCCGCGGTC
2101     TCCTGCGTCT TCCTGGCCTT CGTCATCGTG CTGCTGGCGC ACAGGCTGCG GCGCTGGCAC
2161     AAGTCACGTC TGCTACAGGC TTCGGGAGGC GGCTTAGCGA GCATGCCCGG TTCGCACTTT
2221     GTGGGCGTGG ACGGGGTTCG GGCTTTCCTG CAGACCTATT CCCACGAGGT CTCCCTCACT
2281     GCGGACTCGC GGAAGAGCCA CCTGATTTTC CCCCAGCCCA ACTATGCGGA CACACTCATC
2341     AGCCAGGAGA GCTGTGAGAA AAAGGGTTTT CTATCAGCAC CCCAGTCTTT ACTTGAAGAC
2401     AAAAAGGAAC CATTTTCTCA GGTAAACTTT TGTGATGAAT GTATCAGCTA TCTAGAGAAA
2461     AATAATTCTT GA

Quality Control:cDNA sequencing results is available upon request.