Human PCDH19 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13405-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

PCDH19 cDNA Clone Datasheet

PCDH19 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001105243.1
cDNA Size:	3306
Gene Synonym:	EFMR; EIEE9; PCDH19
Source:	Human

PCDH19 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens protocadherin 19 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 279 C/T and 1470 C/T not causing the amino acid variation.

PCDH19 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PCDH19 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PCDH19 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGTCGC TCCTGCTGCC GGTGCTGCTG CTGCTGGCCA TACTGTGGAC GCAGGCTGCC
61       GCCCTCATTA ATCTCAAGTA CTCGGTAGAA GAGGAGCAGC GCGCCGGGAC GGTGATTGCC
121      AACGTGGCCA AAGACGCGCG AGAGGCGGGC TTCGCGCTGG ACCCCCGGCA GGCTTCAGCC
181      TTTCGCGTGG TGTCCAACTC GGCTCCACAC CTAGTGGACA TCAATCCCAG CTCTGGCCTG
241      CTGGTCACCA AGCAGAAGAT TGACCGTGAT CTGCTGTGTC GCCAGAGCCC CAAGTGCATC
301      ATCTCGCTCG AGGTCATGTC CAGCTCAATG GAAATCTGCG TGATAAAGGT GGAGATCAAG
361      GACCTGAACG ACAATGCGCC CAGTTTCCCG GCAGCACAGA TCGAGCTGGA GATCTCGGAG
421      GCAGCCAGCC CTGGCACGCG CATCCCGCTG GACAGCGCTT ACGATCCAGA CTCAGGAAGC
481      TTTGGCGTGC AGACTTACGA GCTCACGCCC AACGAGCTGT TCGGCCTGGA GATCAAGACG
541      CGCGGCGACG GCTCCCGCTT TGCCGAACTC GTGGTGGAAA AGAGCCTGGA CCGCGAGACG
601      CAGTCGCACT ACAGCTTCCG AATCACTGCG CTAGACGGTG GCGACCCGCC GCGCCTGGGC
661      ACCGTTGGCC TTAGTATCAA GGTGACCGAC TCCAATGACA ACAACCCGGT GTTTAGCGAG
721      TCCACCTACG CGGTGAGCGT GCCAGAAAAC TCGCCTCCCA ACACACCCGT CATCCGCCTC
781      AACGCCAGCG ATCCAGACGA GGGCACCAAC GGCCAGGTGG TCTACTCCTT CTATGGCTAC
841      GTCAACGACC GCACGCGCGA GCTCTTTCAG ATCGACCCGC ACAGTGGCCT GGTCACTGTC
901      ACTGGCGCTT TAGACTACGA AGAGGGGCAC GTGTACGAAC TGGACGTGCA GGCTAAGGAC
961      TTGGGGCCCA ATTCCATCCC GGCACACTGC AAGGTCACCG TCAGCGTGCT GGACACCAAT
1021     GACAATCCGC CGGTCATCAA CCTGCTGTCA GTCAACAGTG AGCTTGTGGA GGTCAGCGAG
1081     AGCGCCCCCC CGGGCTACGT GATCGCCTTG GTGCGGGTGT CTGATCGCGA CTCAGGCCTC
1141     AATGGACGTG TGCAGTGCCG TTTGCTGGGC AATGTGCCCT TTCGACTGCA GGAATATGAG
1201     AGCTTCTCCA CTATTCTGGT GGACGGACGG CTGGACCGCG AGCAGCACGA CCAATACAAC
1261     CTCACAATTC AGGCACGCGA CGGCGGCGTG CCCATGCTGC AGAGTGCCAA GTCCTTTACC
1321     GTGCTCATCA CTGACGAAAA TGACAACCAC CCGCACTTTT CCAAGCCCTA CTACCAGGTC
1381     ATTGTGCAGG AGAACAACAC GCCTGGCGCC TATCTGCTCT CTGTGTCTGC TCGCGACCCC
1441     GACCTGGGTC TCAACGGCAG TGTCTCCTAT CAGATCGTGC CGTCGCAGGT GCGGGACATG
1501     CCTGTCTTCA CCTATGTCTC CATCAATCCC AACTCAGGCG ACATCTACGC GCTGCGATCC
1561     TTTAACCACG AGCAGACCAA GGCGTTCGAA TTCAAGGTGC TGGCCAAGGA CGGCGGCCTT
1621     CCCTCACTGC AAAGCAACGC TACGGTGCGG GTCATCATCC TCGACGTCAA CGACAACACC
1681     CCGGTCATCA CAGCCCCACC TCTGATTAAC GGCACTGCCG AGGTCTACAT ACCCCGCAAC
1741     TCTGGCATAG GCTACCTGGT GACTGTTGTC AAGGCAGAAG ACTACGATGA GGGCGAAAAT
1801     GGCCGAGTCA CCTACGACAT GACCGAGGGC GACCGCGGCT TCTTTGAAAT AGACCAGGTC
1861     AATGGCGAAG TCAGAACCAC CCGCACCTTC GGGGAGAGCT CCAAGTCCTC CTATGAGCTT
1921     ATCGTGGTGG CTCACGACCA CGGCAAGACA TCTCTCTCTG CCTCTGCTCT CGTCCTAATC
1981     TACTTGTCCC CTGCTCTCGA TGCCCAAGAG TCAATGGGCT CTGTGAACTT GTCCTTGATT
2041     TTCATTATTG CCCTGGGCTC CATTGCGGGC ATCCTCTTTG TAACTATGAT CTTCGTGGCA
2101     ATCAAGTGCA AGCGAGACAA CAAAGAGATC CGGACCTACA ACTGCAGAAT TGCTGAGTAC
2161     TCCTATGGGC ATCAAAAGAA ATCAAGCAAG AAGAAAAAAA TCAGTAAGAA TGACATCCGC
2221     CTGGTACCCC GGGATGTGGA GGAGACAGAC AAGATGAACG TTGTCAGTTG CTCTTCCCTG
2281     ACCTCCTCCC TCAACTATTT TGACTACCAC CAGCAGACGC TGCCCCTGGG CTGCCGCCGC
2341     TCTGAGAGCA CTTTCCTGAA TGTGGAGAAC CAGAATACCC GCAACACCAG TGCTAACCAC
2401     ATCTACCATC ACTCTTTCAA CAGCCAGGGG CCCCAGCAGC CTGACCTGAT TATCAACGGT
2461     GTGCCTCTGC CTGAGACTGA AAACTATTCT TTTGACTCCA ACTACGTGAA TAGCCGAGCC
2521     CATTTAATCA AGAGCAGCTC CACCTTCAAG GACTTAGAGG GCAACAGCCT GAAGGATAGT
2581     GGACATGAGG AGAGTGACCA AACTGACAGT GAGCATGATG TCCAGCGGAG CCTGTATTGT
2641     GATACTGCTG TCAACGATGT GCTGAACACC AGTGTGACCT CCATGGGATC TCAGATGCCT
2701     GATCATGATC AGAATGAAGG ATTTCATTGC CGGGAAGAAT GCCGGATTCT TGGCCACTCT
2761     GACAGGTGCT GGATGCCCCG GAACCCCATG CCCATCCGTT CCAAGTCCCC TGAGCATGTG
2821     AGGAACATCA TCGCGCTGTC TATTGAAGCT ACTGCTGCTG ATGTCGAGGC TTATGACGAC
2881     TGCGGCCCCA CCAAACGGAC TTTCGCAACC TTTGGGAAAG ATGTCAGCGA CCACCCGGCT
2941     GAGGAGAGGC CTACCCTGAA AGGCAAGAGG ACTGTCGATG TGACCATCTG CAGCCCCAAG
3001     GTCAACAGCG TTATCCGGGA GGCAGGCAAT GGCTGTGAGG CGATTAGCCC TGTCACCTCC
3061     CCCCTCCACC TCAAGAGCTC TCTGCCCACC AAGCCTTCCG TGTCTTACAC CATTGCCCTG
3121     GCTCCCCCAG CCCGTGATCT GGAGCAGTAT GTCAACAATG TCAACAATGG CCCTACTCGT
3181     CCCTCTGAAG CTGAGCCCCG TGGAGCTGAT AGCGAGAAAG TCATGCATGA GGTCAGCCCC
3241     ATTCTGAAGG AAGGTCGCAA CAAAGAGTCC CCTGGTGTGA AGCGTCTGAA GGATATCGTT
3301     CTCTAA

Quality Control:cDNA sequencing results is available upon request.