PCBP1

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.

PCBP1 Related Areas

 

PCBP1 Alternative Names

PCBP1, hnRNP-E1, HNRPE1, HNRPX, hnRNP-X, alpha-CP1 [Homo sapiens]

Pcbp1, hnRNP E1, hnRNP-E1, WBP17, [a]CP-1, alphaCP-1, alpha-CP1 [Mus musculus]

Summaries for PCBP1

Entrez Gene summary for PCBP1:

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability

OMIM - description for PCBP1:

When expressed with a vaccinia virus system in transformed amnion cells, Leffers et al. (1995) found that both PCBP1 and PCBP2 bound poly(rC) when not phosphorylated; phosphorylated protein bound with much lower affinity. By yeast 2-hybrid analysis of a human brain cDNA library, Kosturko et al. (2006) found that mouse Hnrnpa2 (600124) interacted with human HNRNPE1. They confirmed the interaction with in vivo and in vitro protein interaction assays. Hnrnpe1 colocalized with Hnrnpa2 and A2RE mRNA in granules in dendrites of rat oligodendrocytes. Overexpression of HNRNPE1 or microinjection of exogenous HNRNPE1 in rat neural cells inhibited translation of A2RE mRNA, but not translation of mutated A2RE mRNA. Excess HNRNPE1 added to an in vitro translation system reduced translation efficiency of A2RE mRNA in an Hnrnpa2-dependent manner. Kosturko et al. (2006) hypothesized that binding of HNRNPE1 to HNRNPA2 inhibits A2RE mRNA translation during granule transport.

Wikipedia summary for PCBP1:

Poly(rC)-binding protein 1 is a protein that in humans is encoded by the PCBP1 gene. This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability

Human PCBP1 Protein General Information

 

• Protein names: Submitted name: Poly(RC) binding protein 1
• Sequence length: 356 AA.
• Domain: Repeat
• Sequence similarities: Contains 3 KH domains.
• Post-translational modification: Phosphorylated; lowers poly(rC)-binding activity.
• Subcellular location: Nucleus. Cytoplasm. Note: Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
• Tissue specificity: Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

General information above from UniProt

Function for PCBP1 Protein

UniProtKB:

Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.

Genatlas:

• PCBP1 function as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES
• regulating the translation of reticulocyte 15-lipoxygenase (ALOX15) mRNA
• PCBP1 promotes poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure
• PCBP1 is implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA
• PCBP1 plays a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability
• PCBP1 can function as a cytosolic iron chaperone in the delivery of iron to ferritin

Homology for human PCBP1

• ortholog to murine Pcbp1
• homolog to drosophila mub
• homolog to C.elegans Y119D3B.17 and F26B1.2