Mouse PBK/TOPK Gene cDNA Clone / ORF Clone

PBK/TOPK cDNA Clone Datasheet

PBK/TOPK cDNA Clone Product Information

• Gene Bank Ref.ID: NM_023209.2
• cDNA Size: 993
• Gene Synonym: TOPK; AW538537; D14Ertd732e; 2810434B10Rik; Pbk
• Source: Mouse

PBK/TOPK cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus PDZ binding kinase DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

PBK/TOPK cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

PBK/TOPK cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

PBK/TOPK cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAAGGAA TTAATAATTT CAAGACGCCA AACAAATCTG AAAAAAGGAA ATCTGTATTA
61       TGTTCCACTC CATGTGTAAA TATCCCTGCC TCTCCATTTA TGCAGAAGCT TGGCTTTGGG
121      ACTGGGGTCA GCGTTTACCT AATGAAAAGA TCTCCAAGAG GGTTGTCTCA TTCTCCTTGG
181      GCCGTGAAAA AGATAAGTCT TTTATGCGAT GATCATTATC GAACTGTGTA TCAGAAGAGA
241      CTAACTGATG AAGCTAAGAT TTTAAAAAAC CTTAATCACC CAAACATTAT AGGATATCGT
301      GCTTTTACTG AAGCCAGTGA TGGTAGTCTG TGCCTTGCTA TGGAGTATGG AGGTGAAAAG
361      TCTCTGAATG ACTTAATAGA AGAGCGGAAC AAAGACAGTG GAAGTCCTTT TCCAGCAGCT
421      GTAATTCTCA GAGTTGCTTT GCACATGGCC AGAGGGCTAA AGTACCTGCA CCAAGAAAAG
481      AAGCTGCTTC ATGGAGACAT AAAGTCTTCA AATGTTGTAA TTAAAGGTGA TTTTGAAACA
541      ATTAAAATCT GTGATGTAGG AGTCTCTCTG CCATTGGATG AAAATATGAC TGTGACTGAT
601      CCTGAGGCCT GTTATATTGG TACTGAGCCA TGGAAACCCA AGGAAGCGTT GGAAGAAAAT
661      GGCATCATTA CTGACAAGGC AGATGTGTTT GCTTTTGGCC TTACTCTGTG GGAAATGATG
721      ACTTTATGTA TTCCACACGT CAATCTTCCA GATGATGATG TTGATGAAGA TGCAACCTTT
781      GATGAGAGTG ACTTCGATGA TGAAGCATAT TATGCAGCTC TGGGGACAAG GCCATCCATC
841      AACATGGAAG AGCTGGATGA CTCCTACCAG AAGGCCATTG AACTCTTCTG TGTGTGCACT
901      AATGAGGATC CTAAAGATCG CCCGTCTGCT GCACACATCG TTGAAGCTTT GGAACTAGAT
961      GGCCAATGTT GTGGTCTAAG CTCAAAGCAT TAA

 

Quality Control:cDNA sequencing results is available upon request.