Anti-PBK Antibody

Cat: 10729-MM05
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Anti-PBK Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-PBK Antibody
Validated applications
WB,ELISA,ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human PBK
Immunogen
Recombinant Human PBK protein (Catalog#10729-H08B)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PBK / TOPK (rh PBK; Catalog#10729-H08B; NP_060962.2; Met 1-Val 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #1E5E5E6
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-PBK Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
ELISA(Det) 1:5000-1:50000 This antibody will detect Human PBK / TOPK in ELISA pair set (Catalog: # SEK10729). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10729-MM10).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-PBK Antibody (Mouse Monoclonal antibody) Images
Human PBK/TOPK Western blot (WB) 13266

Anti-PBK mouse monoclonal antibody at 1:500 dilution

Lane A: SW480 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:36 kDa

Observed band size:38 kDa

Anti-PBK Antibody Alternative Names
Anti-CT84 Antibody;Anti-HEL164 Antibody;Anti-Nori-3 Antibody;Anti-SPK Antibody;Anti-TOPK Antibody
PBK Background Information

PDZ binding kinase (PBK), also known as TOPK (T-LAK cell-originated protein kinase), is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family, and has all the characteristic protein kinase subdomains and a C-terminal PDZ-binding T/SXV motif. PBK is expressed in the testis restrictedly expressed in outer cell layer of seminiferous tubules, as well as placenta. PBK may be enrolled in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis.This mitotic kinase phosphorylates MAP kinase p38 and seems to be active in mitosis. When phosphorylated, PBK forms a protein-protein interaction with tumor suppressor p53 (TP53), leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. The expression level of PBK is thus upregulated in a variety of neoplasms including hematological malignancies.

Full Name
PDZ binding kinase
References
  • Zakut-Houri R., et al.,(1985), Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells. EMBO J. 4:1251-1255.
  • Lamb P., et al., (1986), Characterization of the human p53 gene.Mol. Cell. Biol. 6:1379-1385.
  • Harlow E., et al.,(1985), Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.Mol. Cell. Biol. 5:1601-1610.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-PBK Antibody Rabbit Polyclonal ELISA 10729-RP02
    Anti-PBK Antibody Mouse Monoclonal ELISA(Cap) 10729-MM10
    Anti-PBK Antibody Rabbit Polyclonal ELISA 10729-RP01
    info info
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