PARVA / Actopaxin Protein (GST Tag)

Parvin, alpha Protein Datasheet

PARVA / Actopaxin Protein Product Information

• Synonym: PARVA, MXRA2
• Protein Construction: A DNA sequence encoding the mature form of human PARVA(Q9NVD7-1) (Met1-Glu372) was fused with the GST tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

PARVA / Actopaxin Protein QC Testing

• Purity: > 60 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human PARVA /GST chimera consists of 606 amino acids and has a predicted molecular mass of 69.4 kDa. It migrates as an approximately 69 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PARVA / Actopaxin protein

PARVA / Actopaxin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PARVA / Actopaxin Protein Description

Actopaxin, also known as alpha-parvin, is a member of the parvin family. It contains 2 CH (calponin-homology) domains and is widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. It interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Actopaxin probably plays a role in the regulation of cell adhesion and cytoskeleton organization. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. Actopaxin also exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. It is absent from actin-rich cell-cell adherens junctions.

References

1. Korenbaum E. et al., 2002, Gene. 279 (1): 69-79.
2. Nikolopoulos SN. et al., 2002, J Biol Chem. 277 (2): 1568-75.
3. Tu Y. et al., 2001, J Cell Biol. 153 (3): 585-98.