Mouse PARP1 / PARP-1 Protein (His Tag)

Poly (ADP-ribose) polymerase family, member 1 Protein Datasheet

PARP1 / PARP-1 Protein Product Information

• Synonym: 5830444G22Rik, AI893648, Adprp, Adprt1, C80510, PARP, PPOL, parp-1, sPARP-1
• Protein Construction: A DNA sequence encoding the mouse PARP1 (NP_031441.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the N-terminus.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

PARP1 / PARP-1 Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized mouse PARP1 at 10 μg/mL (100 μl/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.021 μg/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse PARP1 consists of 1033 amino acids and has a calculated molecular mass of 115 KDa. It migrates as an approximately 75KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH8.0, 10%gly, 0.1mM TCEP
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: PARP1 / PARP-1 protein

PARP1 / PARP-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

PARP1 / PARP-1 Protein Description

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

References

1. Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
2. Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
3. Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
4. Ahell I. et al., 2008, Nature. 451: 81-85.